Tungstate as complex-forming reagent facilitating separation of selected polyphenols by capillary electrophoresis and its comparison with borate

Electrophoresis
Pavel JácLenka Kaderová

Abstract

A novel electrophoretic BGE containing tungstate as complex-forming reagent is suitable for the separation of polyphenols. Similar to molybdate-containing BGE reported earlier (cf. M. Polásek, et al.., Talanta 2006, 69, 192) addition of tungstate to BGE affects significantly migration of compounds/ligands with vicinal -OH groups due to the formation of negatively charged complexes involving W(VI) as central ion. Baseline separation of mixtures of flavonoids (apigenin, luteolin, hyperoside, quercetin, and rutin) and phenolic acids (chlorogenic and p-coumaric acid) was achieved within 15 min with optimized BGE of pH 7.4 containing 50 mM N-(2-hydroxyethyl)piperazine-2'-(2-ethanesulfonic acid) (HEPES), 2.2 mM tungstate, and 25% v/v of methanol. The separation was performed in a 75 cm (effective length 42 cm)x 75 microm id uncoated fused-silica capillary at 30 kV with spectrophotometric detection at 275 nm. The calibration curves were rectilinear for 25-175 microg/mL of all analytes (cinnamic acid as the internal standard). The LODs ranged from 1.8 to 6 microg/mL for all analytes except for chlorogenic acid. Intraday precision (n = 6) of migration times (RSD < or = 1.2%) and peak areas (RSD < or = 5.6%) was evaluated. The tungstate-...Continue Reading

References

Dec 16, 2000·Carbohydrate Research·M Luísa RamosV M Gil
Jan 30, 2002·Journal of Pharmaceutical and Biomedical Analysis·Leena Suntornsuk
Apr 7, 2004·Journal of Chromatography. a·Shu-Ping Wang, Kuo-Jun Huang
Jan 18, 2006·Journal of Pharmaceutical and Biomedical Analysis·Pavel JácMarie Pospísilová
Aug 31, 2006·Journal of Agricultural and Food Chemistry·Yi LiuPengfei Tu
Sep 14, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Igor G ZenkevichViktor A Utsal

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Citations

May 4, 2010·Analytical and Bioanalytical Chemistry·Leena Suntornsuk
Dec 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Roberto Gotti
Dec 6, 2011·Electrophoresis·Xiao-jia ChenShao-Ping Li
Dec 7, 2016·Electrophoresis·Baljinder SinghAshok Kumar Malik
Jun 15, 2017·BioFactors·Joanna KarpinskaWłodzimierz Lewandowski
May 26, 2010·Analytical Chemistry·Nicholas W FrostMichael T Bowser

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