Tungsten and molybdenum heteropolyacids as staining and contrasting agents: reactivity with epoxyresin-embedded cell and tissue structures.

Acta histochemica
J C StockertM C Risueño

Abstract

In this work, we carry out a further approach to the knowledge of the reaction mechanism of phosphotungstic and phosphomolybdic acids (PTA and PMA), as well as some derivatives, with cell structures from epoxyresin-embedded materials. Applied on thin sections from glutaraldehyde-fixed tissues, PTA and PMA induced a strong electron contrasting reaction in spermatid acrosomes, goblet cell mucin, callose and plant cell walls, endexine, intine and starch granules. In light microscopy, the localization of heteropolyacids on these structures was achieved by treatments of semithin sections with suitable reducing agents (titanous sulfate, stannous chloride, sodium borohydride, or p-phenylenediamine) to form the mixed-valence heteropolyblues, or with Schiffs's reagent. The use of PTA-dye complexes (pyronin-PTA and Mallory's PTA-hematoxylin) also showed the same staining pattern. Taking into account the chemical characteristics of the PTA- and PMA-reactive tissue elements, the present results indicate that heteropolyacids selectively enter into the highest hydrophilic structures from non-polar epoxy-embedded sections; after brief washing, they appear predominantly retained in tissue structures containing a great amount of carbohydrate co...Continue Reading

References

Oct 1, 1986·Journal of Microscopy·M L MoleroJ C Stockert
Jan 1, 1988·Gynecologic and Obstetric Investigation·W I van der MeijdenW C de Bruijn
Nov 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G QuintarelliJ A Cifonelli
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Citations

Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A TatoJ M Ferrer
Oct 23, 2010·Ecotoxicology and Environmental Safety·Nikolay Strigul

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