Tungsten-enhanced growth of Methanosphaera stadtmanae.

BMC Research Notes
Bédis DridiMichel Drancourt

Abstract

The methanogenic Archaea Methanosphaera stadtmanae has been detected in the human gut microbiota by both culture and culture-independent methods. Its growth reaches an exponential phase after 5 to 7-day culture in medium 322 (10% vol). Our recent successful isolation of Methanomassiliicoccus luminyensis, a tungstate-selenite-requiring Archaea sharing similar metabolism characteristics with M. stadtmanae prompted us to study the effects of tungsten and selenium on M. stadtmanae growth. Addition of 0.2 mg/L sodium tungstate to medium 322 yielded, 48 hours after inoculation, a growth rate equivalent to that obtained after 6 days with control culture as measured by methane monitoring and optical density measurement. Addition of 50 μg/mL sodium selenate had no effect on M. stadtmanae growth. Quantitative real-time PCRs targeting the M. stadtmanae 16S rRNA confirmed these data. These data provide new information regarding the poorly known nutritional requirements of the human gut colonizing organismsM. stadtmanae. Adding sodium tungstate to basal medium may facilitate phenotypic characterization of this organism and additionally aid the isolation of new Archaea from complex host microbiota.

References

Jun 1, 1977·Journal of Bacteriology·J B Jones, T C Stadtman
Nov 1, 1986·Applied and Environmental Microbiology·N BelayL Daniels
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W M ChingT C Stadtman
Aug 6, 2009·International Journal of Systematic and Evolutionary Microbiology·Kirill V KrivushinElizaveta M Rivkina
Mar 23, 2011·Anaerobe·Bédis DridiMichel Drancourt
Jun 18, 2011·The Journal of Antimicrobial Chemotherapy·Bédis DridiMichel Drancourt
Aug 4, 2012·International Journal of Systematic and Evolutionary Microbiology·Bédis DridiMichel Drancourt

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Citations

Apr 25, 2013·PloS One·Saber KhelaifiaMichel Drancourt
Jul 22, 2015·Environmental Science and Pollution Research International·Cécile MilitonPatricia Bonin
Jul 31, 2016·Journal of Bioscience and Bioengineering·Guang-Chao YangBo-Zhong Mu
Feb 16, 2018·Future Microbiology·Elodie GuilhotGrégory Dubourg
Jan 9, 2015·Clinical Microbiology Reviews·Jean-Christophe LagierDidier Raoult
Apr 7, 2021·Probiotics and Antimicrobial Proteins·Nisha ChuphalVikas Kumar

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Methods Mentioned

BETA
PCR

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