Tuning capillary surface properties by charged polymeric coatings

Journal of Chromatography. a
Laura Sola, M Chiari

Abstract

Separation of proteins in capillary electrophoresis (CE) is often hindered by their interactions with the silanols groups on the inner surface of the fused silica capillary. In particular, the positive charges of alkaline proteins establish electrostatic interactions with the negative charges of the surface, leading to peak broadening and loss of separation efficiency. Moreover, uncontrolled electroosmotic flow (EOF), severely impacts on analyte mobility causing either loss of resolution or loss of efficiency. Among several strategies, coatings are widely employed to mask capillary surface silanols and so to reduce protein adsorption and EOF. Here we report on the synthesis and characterization of a novel family of adsorbed polymeric coatings, which provide improved performance in terms of prevention of protein adsorption and EOF regulation. In particular, we have added to the polymer backbone, made of N, N-dimethylacrylamide, different ionizable monomers (weak acrylamido acids and bases commercially available with the trade name of Immobilines) to confer a net positive or negative charge to the polymer chain depending on the buffer pH. As a consequence, the separation of alkaline protein is drastically improved in different pH...Continue Reading

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May 9, 2012·Journal of Chromatography. a·Mei HeD Jed Harrison
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Dec 4, 2012·Journal of Chromatography. a·Laura Sola, Marcella Chiari
Oct 15, 2013·Electrophoresis·Shuai Sherry Zhao, David D Y Chen

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Citations

Aug 9, 2016·Analytica Chimica Acta·Sille Štěpánová, Václav Kašička
Jul 2, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Chiara FinettiMarcella Chiari
Aug 13, 2017·Electrophoresis·Yuanyuan LiuChao Yan

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