Tuning of SlARF10A dosage by sly-miR160a is critical for auxin-mediated compound leaf and flower development

The Plant Journal : for Cell and Molecular Biology
Subha DamodharanTzahi Arazi

Abstract

miR160 adjusts auxin-mediated development by post-transcriptional regulation of the auxin response factors ARF10/16/17. In tomato, knockdown of miR160 (sly-miR160) suggested that it is required for auxin-driven leaf blade outgrowth, but whether additional developmental events are adjusted by sly-miR160 is not clear. Here, the SlMIR160 genes and the genes of its SlARFs targets were edited by CRISPR/Cas9 resulting in the isolation of loss-of-function mutants. In addition, hypomorphic mutants that accumulate variable reduced levels of sly-miR160a were isolated. We found that the loss-of-function mutants in SlMIR160a (CR-slmir160a-6/7) produced only four wiry leaves, whereas the hypomorphic mutants developed leaves and flowers with graded developmental abnormalities. Phenotypic severity correlated with the upregulation of SlARF10A. Consistent with that, double mutants in SlMIR160a and SlARF10A restored leaf and flower development indicating that over-accumulation of SlARF10A underlay the developmental abnormalities exhibited in the CR-slmir160a mutants. Phenotype severity also correlated with the upregulation of the SHOOT MERISTEMLESS homolog Tomato Knotted 2, which in turn activated the transcription of the cytokinin biosynthesis ...Continue Reading

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Citations

Nov 21, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shuai SunXia Cui
Apr 3, 2020·Plant Signaling & Behavior·Bhavani Natarajan, Anjan K Banerjee
Aug 19, 2020·Nature Plants·Alon IsraeliNaomi Ori
Apr 4, 2021·International Journal of Molecular Sciences·Mengyi ChenYanwei Hao
May 1, 2021·International Journal of Molecular Sciences·Wanping LinBen Spitzer-Rimon

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