Tuning protein assembly pathways through superfast amyloid-like aggregation

Biomaterials Science
Chen LiPeng Yang

Abstract

Amyloid formation of proteins is not only relevant for neurodegenerative diseases, but has recently emerged as a groundbreaking approach in materials science and biotechnology. However, amyloid aggregation of proteins in vitro generally requires a long incubation time under extremely harsh conditions, and the understanding of the structural motif to determine amyloid assembly is extremely limited. Herein we reveal that the integration of three important building blocks in typical globular proteins is crucial for superfast protein amyloid-like assembly including the segment required for high fibrillation propensity, abundant α-helix structures and intramolecular S-S bonds to lock the α-helix. With the reduction of the S-S bond by tris(2-carboxyethyl)phosphine (TCEP), the α-helix was rapidly unlocked from the protein chain, and the resultant unfolded monomer underwent a fast transition to β-sheet-rich amyloid oligomers and protofibrils in minutes, which further assembled into a macroscopic nanofilm at the air/water interface and microparticles in bulk solution, respectively.

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Citations

Jun 26, 2020·Advanced Materials·Dong WangPeng Yang
Jul 22, 2020·Biomaterials Science·Juanhua TianPeng Yang
Dec 12, 2018·Advanced Materials·Rongrong QinPeng Yang
Feb 13, 2018·Biomaterials Science·Chen LiPeng Yang
Jul 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jian ZhaoPeng Yang
Jan 23, 2021·Journal of Colloid and Interface Science·Mohammadhasan HedayatiMatt J Kipper
Mar 30, 2021·International Journal of Biological Macromolecules·Dong WangYingwei Huang
Apr 10, 2021·International Journal of Biological Macromolecules·Fei LiTao Wang
Jul 23, 2021·Journal of Colloid and Interface Science·Xiaomin YeFangfu Ye
Jul 21, 2021·Accounts of Chemical Research·Yongchun LiuPeng Yang
Dec 18, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Fei TaoPeng Yang
May 31, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Kyle YuYi Y Zuo
Mar 23, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Lu XuYi Y Zuo

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Methods Mentioned

BETA
circular dichroism
AFM

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