Tuning the transport properties of HIV-1 Tat arginine-rich motif in living cells

Traffic
Francesco CardarelliFabio Beltram

Abstract

A large body of work is currently devoted to the rational design of new molecular carriers for the controlled delivery of cargoes (e.g. proteins or nucleic acids) to relevant subcellular domains, particularly the nucleus. In this article, we show that rational mutagenesis of the human immunodeficiency virus type 1 Tat-derived peptide (YGRKKRRQRRR) affords variants with finely tuned intercompartmental dynamics and controllable transport mechanisms. Our findings are made possible by the demonstration that the Tat peptide possesses two competing functionalities capable of active nuclear targeting and additional binding to intracellular moieties. By altering the competition between these two functions, we show how to control cargo localization of Tat peptide chimeras. Our investigation provides a unified, coherent description of previous conflicting in vivo and in vitro results and lets the true nature of the Tat peptide emerge.

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Citations

Nov 27, 2008·Analytical and Bioanalytical Chemistry·Michela SerresiFabio Beltram
Nov 27, 2008·Analytical and Bioanalytical Chemistry·Ranieri BizzarriFabio Beltram
May 16, 2012·Analytical and Bioanalytical Chemistry·Ranieri BizzarriFabio Beltram
Mar 12, 2013·Nature Medicine·Gangwen HanXiao-Jing Wang
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eduardo F FariasSamuel Waxman
Oct 28, 2009·The Journal of Biological Chemistry·Francesco CardarelliFabio Beltram
Feb 16, 2011·The Journal of Biological Chemistry·Francesco CardarelliFabio Beltram
Aug 23, 2011·PloS One·Mahdi SarmadyAydin Tozeren
Nov 21, 2012·PloS One·Mohamed Ali JarbouiVirginie W Gautier
Jun 27, 2014·PloS One·Paolo BianchiniRanieri Bizzarri
Oct 29, 2015·Cellular and Molecular Life Sciences : CMLS·Yana R MusinovaYegor S Vassetzky
Dec 15, 2015·Biosensors & Bioelectronics·M F FatinSubash C B Gopinath
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Jun 18, 2015·Frontiers in Microbiology·Wahyu N WulanReena Ghildyal
Apr 13, 2018·Physical Chemistry Chemical Physics : PCCP·Yeongchang GohKang Taek Lee
Mar 6, 2019·Medical Microbiology and Immunology·Cassandra SpectorMichael R Nonnemacher
Apr 26, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sonia MediouniSusana T Valente
Sep 29, 2011·Traffic·Solène DebaisieuxBruno Beaumelle
Sep 1, 2011·Biophysics Reviews·Elizabeth Hinde, Francesco Cardarelli
Apr 17, 2010·Molecular Pharmaceutics·Lorenzo AlbertazziFabio Beltram

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