Tuning up microbiome analysis to monitor WWTPs' biological reactors functioning.

Scientific Reports
Miguel de CelisAntonio Santos

Abstract

Wastewater treatment plants (WWTPs) are necessary to protect ecosystems quality and human health. Their function relies on the degradation of organic matter and nutrients from a water influent, prior to the effluent release into the environment. In this work we studied the bacterial community dynamics of a municipal WWTP with a membrane bioreactor through 16S rRNA gene sequencing. The main phyla identified in the wastewater were Proteobacteria, Bacteroidetes, Chloroflexi, Planctomycetes and Actinobacteria. The WWTP is located in Spain and, like other studied WWTP in temperate climate zones, the temperature played a major role in community assembly. Seasonal community succession is observed along the two years sampling period, in addition to a continual annual drift in the microbial populations. The core community of the WWTP bioreactor was also studied, where a small fraction of sequence variants constituted a large fraction of the total abundance. This core microbiome stability along the sampling period and the likewise dissimilarity patterns along the temperature gradient makes this feature a good candidate for a new process control in WWTPs.

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Aug 5, 2010·Applied Microbiology and Biotechnology·Ting-ting RenXiao-yan Li
Sep 4, 2010·Environmental Science & Technology·Siqing XiaSlawomir W Hermanowicz
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Oct 12, 2013·Journal of Industrial Microbiology & Biotechnology·Ana Lusia LealVictor Hugo Valiati
Apr 5, 2014·PLoS Computational Biology·Paul J McMurdie, Susan Holmes
Nov 22, 2015·Applied and Environmental Microbiology·Louise FeldChristian Nyrop Albers
Mar 5, 2016·World Journal of Microbiology & Biotechnology·Agnieszka Cydzik-Kwiatkowska, Magdalena Zielińska
May 24, 2016·Nature Methods·Benjamin J CallahanSusan P Holmes
Oct 6, 2016·PeerJ·Christina A KelloggSandra D Brooke
Jul 22, 2017·The ISME Journal·Benjamin J CallahanSusan P Holmes
Oct 22, 2017·The Science of the Total Environment·Tarik AbzazouRosa Mª Araujo
Mar 20, 2018·Biotechnology Advances·Yu XiaYunfeng Yang
Mar 16, 2019·Scientific Reports·Juliet JohnstonSebastian Behrens

❮ Previous
Next ❯

Citations

Nov 24, 2020·The Science of the Total Environment·Vedran VučićSusanne Günther
Dec 29, 2020·Computational and Structural Biotechnology Journal·Nicholas A BokulichBenjamin D Kaehler

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

phyloseq
HTS
Silva
DADA2
vegan
Tax4Fun
R
PERMANOVA

Related Concepts

Related Feeds

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.