PMID: 11341373May 9, 2001Paper

Turbidimetric and HPLC assays for the determination of formulated lysozyme activity

The Journal of Pharmacy and Pharmacology
Y H LiaoG P Martin

Abstract

In several studies lysozyme has been employed as a model protein to investigate the effects of formulation factors upon biological activity. The aim of this work was to develop and validate an HPLC technique to assay lysozyme and to compare the results with biological activity determined from a validated turbidimetric assay. The turbidimetric assay was based upon the lytic action of lysozyme on Micrococcus lysodeikticus cells, whilst the reverse-phase HPLC assay employed an acetonitrile gradient in 0.1% trifluoroacetic acid. The limits of detection and quantification were 3.84 and 6.24 microg mL(-1) for HPLC assay, whilst the corresponding values for turbidimetric assay were 1.94 and 3.86 microg mL(-1). The methods were used to monitor the loss of enzyme activity after heating. Lysozyme concentrations determined from HPLC peak height were found to correlate (r2 = 0.9963) with those obtained from turbidimetric assay.

References

Nov 1, 1989·Pharmaceutical Research·M C ManningR T Borchardt
Jan 1, 1971·Analytical Biochemistry·G GorinL Papapavlou
Jan 1, 1983·Methods of Biochemical Analysis·N Grossowicz, M Ariel
Jul 8, 1998·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·F Franks
Jun 1, 1999·Pharmaceutical Research·S BranchuH Nyqvist
Dec 1, 1949·Journal of Bacteriology·A N SMOLELIS, S E HARTSELL

❮ Previous
Next ❯

Citations

May 28, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·G G BuonocoreL Nicolais
Apr 15, 2008·British Poultry Science·O Wellman-LabadieM T Hincke
Jan 15, 2008·Physiological and Biochemical Zoology : PBZ·Olivier Wellman-LabadieMaxwell T Hincke
Nov 8, 2003·The Journal of Pharmacy and Pharmacology·Yong-Hong LiaoGary P Martin
Jul 6, 2012·Pharmaceutical Development and Technology·Wasu WitoonsaridsilpNarong Sarisuta
Oct 27, 2012·Journal of Microencapsulation·Naveen K BejugamMartin J D'Souza
Feb 22, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Olivier Wellman-LabadieMaxwell T Hincke
Jan 13, 2015·International Journal of Pharmaceutics·Milica StankovićWouter L J Hinrichs
Sep 3, 2020·Analytical and Bioanalytical Chemistry·Mohandoss SonaimuthuHui-Fen Wu
Jun 30, 2004·Journal of Food Protection·G G BuonocoreM A Del Nobile
Jun 19, 2019·Analytical and Bioanalytical Chemistry·Mengyao FangJianji Wang
Nov 19, 2009·Journal of Agricultural and Food Chemistry·Nadine SchneiderMonika Pischetsrieder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.