Turbidimetric Method: A Multi-Advantageous Option for Assessing the Potency of Ceftriaxone Sodium in Powder for Injection.

Journal of AOAC International
Mariana Teixeira da TrindadeHérida Regina Nunes Salgado

Abstract

Ceftriaxone sodium, an antimicrobial used in parenteral form, does not have a microbiological method by turbidimetry described in the literature. For drugs from antimicrobial class, the existence of a microbiological method for assessing their potency is essential. Not only are the results from the physical-chemical methods enough, but microbiological analyzes are also necessary. Thus, this paper reports the development and validation of an efficient, accurate, reproducible, fast, and low-cost microbiological assay by turbidimetry to quantify ceftriaxone sodium in powder for injection. Water was used as the diluent to prepare the ceftriaxone solutions. BHI broth as used as culture media for the growth of the S. aureus ATCC 6538 at 9%. The method was linear in the range of 100-196 µg/mL, selective against the sample adjuvants and the forced degradation test, precise (intraday RSD 4.53%, interday RSD 3.85% and between analysts tcalculated 0.14 < 2.23 tcritical), accurate with recovery of 100.33% and robust against minor changes in the volume of culture medium used, wavelength, incubation time, and inoculum concentration. The turbidimetric method developed in this paper is a convenient and valuable alternative to the routine quali...Continue Reading

References

Sep 8, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Alaa S Amin, Gamal H Ragab
Jun 1, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexander KratzerFrieder Kees
Jun 20, 2014·The Journal of Pharmacy and Pharmacology·Ulrich GergsJoachim Neumann
Jan 27, 2015·Journal of Microbiological Methods·Lucélia Magalhães da Silva, Hérida Regina Nunes Salgado
Jul 18, 2015·Pharmaceutics·Eliane Gandolpho Tótoli, Hérida Regina Nunes Salgado
May 1, 2016·Talanta·Mariane Machado Curbete, Hérida Regina Nunes Salgado
Aug 2, 2017·Journal of AOAC International·Ana Carolina Kogawa, Hérida Regina Nunes Salgado
Oct 1, 2013·Journal of Pharmaceutical Analysis·Edith Cristina Laignier Cazedey, Hérida Regina Nunes Salgado
Jan 2, 2020·Talanta·Eliane Gandolpho Tótoli, Hérida Regina Nunes Salgado
Nov 27, 2020·Journal of AOAC International·Patrícia Aleixa Do NascimentoHérida Regina Nunes Salgado

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Citations

Dec 1, 2020·Journal of AOAC International·Patrícia Aleixa do NascimentoHérida Regina Nunes Salgado

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