PMID: 6406768Apr 1, 1983Paper

Turbidometric evaluations of platelet activation: relative contributions of measured shape change, volume, and early aggregation

Journal of Pharmacological Methods
J G Milton, M M Frojmovic

Abstract

The relative contributions of ADP-induced changes in mean platelet axial ratio (R), mean volume (VT), pseudopod formation, refractive index, and early platelet aggregation to the changes in light transmission (%T) through stirred platelet suspensions (aggregometer) are determined. Measurement of the change in R within the first 10s of ADP addition indicates that the slope of the initial decrease in %T (5s) is in good agreement with that predicted from the theory of Latimer et al. (1977). However, when the changes in %T due to changes in VT are also considered, the observed rate of change of %T is shown to be 20-210% larger than predicted. These observations point to an additional process which produces a decrease in %T. It is shown that platelet aggregation occurring on this early time scale does not contribute to the initial changes in %T. Aggregation does lead to an increase in %T once there are greater than or equal to 30-40% singlet platelets aggregated which occurs at greater than or equal to 4-5s for 1-10 microM ADP. In the absence of aggregation, the extent of %T decrease at greater than 40s is greater than 25% smaller than predicted from the change in R and VT. It is suggested that at early times, changes in platelet re...Continue Reading

References

Sep 1, 1976·Biophysical Journal·M M Frojmovic, R Panjwani
Apr 22, 1977·The Journal of Membrane Biology·P LindnerK Rosenheck
Jun 1, 1976·Annals of Biomedical Engineering·H N Chang, C R Robertson
May 1, 1975·Journal of Theoretical Biology·P Latimer
Mar 1, 1976·Microvascular Research·M M FrojmovicH L Goldsmith
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Dec 1, 1968·Journal of Theoretical Biology·P LatimerF D Bryant
Jan 1, 1980·Canadian Journal of Physiology and Pharmacology·J G MiltonM M Frojmovic
Jul 1, 1982·Applied Optics·P Latimer, F Wamble

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Citations

Sep 1, 1986·Basic Research in Cardiology·J NimpfT Kenner
Aug 15, 1988·Experientia·D A Deranleau
Jan 1, 1994·Pharmacology & Therapeutics·P Sargeant, S O Sage
Jun 1, 1987·Journal of Cellular Physiology·A LivneA Rothstein
Oct 22, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·A L LitvinenkoV P Maltsev
Jun 19, 2004·Platelets·Martyn P Mahaut-SmithRichard J Evans
Sep 1, 1987·Thrombosis Research·J G Milton, M M Frojmovic
Mar 23, 2018·International Heart Journal·Chunyang TianZhaoqing Sun
May 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J J ZwagingaJ J Sixma
Jul 1, 1988·European Journal of Haematology·P GroscurthK Rhyner
Nov 1, 1984·Scandinavian Journal of Haematology·P ZillaA von Felten
Jul 30, 1985·Biochimica Et Biophysica Acta·R R Hantgan
Jun 19, 1986·Biochimica Et Biophysica Acta·J A Malinski, G L Nelsestuen
Jun 30, 2021·Analytical Methods : Advancing Methods and Applications·Alena L LitvinenkoValeri P Maltsev

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