Turbot TNFalpha gene: molecular characterization and biological activity of the recombinant protein

Molecular Immunology
M C OrdásB Novoa

Abstract

The tumor necrosis factor (TNF) superfamily is composed by several proteins with similar structure and functions. One of the main representatives of this family is TNF-alpha (TNFalpha), a proinflammatory cytokine which is produced by different immune cells and presents a wide variety of activities. Using the RACE technique, we have cloned and sequenced the turbot TNF cDNA. The analysis of its sequence showed several conserved motifs characteristic of members of the TNFalpha family. A phylogenetic tree constructed with different TNFs of fish and mammals grouped our sequence within the fish TNFalpha cluster. Therefore, the turbot TNF here studied was identified as TNFalpha. The complete TNFalpha gene was obtained by gene walking, and, similarly to the other known fish TNFalpha genes, presented three introns and four exons. A PCR was designed to study the turbot TNFalpha expression in vivo using as stimulus the bacteria Vibrio pelagius strain Hq222 and virus VHSV. The expression of the cytokine happened early after injection, and it was dependent on the pathogen injected and organ analyzed. Virus induced a higher TNFalpha expression, but this response was shorter in time than that induced by bacteria. In addition, TNFalpha express...Continue Reading

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Citations

Jun 22, 2010·Cell and Tissue Research·Nicolò ParrinelloDaniela Parrinello
Sep 17, 2013·Fish & Shellfish Immunology·Tiehui Wang, Christopher J Secombes
Dec 4, 2012·International Journal of Molecular Sciences·Lei GaoWeidong Liu
Aug 22, 2013·Developmental and Comparative Immunology·Hetron Mweemba Munang'anduØystein Evensen
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Dec 29, 2011·Journal of Signal Transduction·Tiehui WangChristopher J Secombes
Dec 4, 2015·Biology·Jordan W HodgkinsonMiodrag Belosevic
Nov 9, 2007·Developmental and Comparative Immunology·Leon GrayferMiodrag Belosevic
Apr 17, 2012·Developmental and Comparative Immunology·Lv-yun ZhuJian-zhong Shao
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Sep 20, 2015·Fish & Shellfish Immunology·Paolo RonzaMaría Isabel Quiroga
Sep 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria ForlenzaGeert F Wiegertjes
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Mar 19, 2011·Developmental and Comparative Immunology·Aja M Rieger, Daniel R Barreda
Apr 30, 2011·Developmental and Comparative Immunology·Gregory D Wiens, Gavin W Glenney

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