Turn-on detection of glutathione S-transferase based on luminescence resonance energy transfer between near-infrared to near-infrared core-shell upconversion nanoparticles and organic dye.

Analytical and Bioanalytical Chemistry
Hongqi ChenLun Wang

Abstract

Glutathione S-transferase (GST) is a detoxification enzyme of the liver and kidney. Based on the toxicological effect of GST, it is of great significance to develop a rapid and sensitive detection method for GST. In this work, a new luminescence resonance energy transfer (LRET) system has been designed to detect glutathione S-transferase in the near-infrared (NIR) region by utilizing NaGdF4:Yb3+,Tm3+@NaYF4 upconversion nanoparticles (UCNPs) as the donor and NIR dye-806@Glutathione (IR806@GSH) as the acceptor. NaGdF4:Yb3+,Tm3+@NaYF4 UCNPs were synthesized by a coprecipitation method and surface modification of NOBF4. The donor (positively charged) interacted with the acceptor (negatively charged) via electrostatic interactions to bring them into close proximity; then, LRET occurred and the luminescence was quenched. In the presence of GST, GST can specifically interact with the GSH of IR806@GSH molecule, making IR806@GSH far away from the donor surface, inhibiting the LRET, and restoring the luminescence of the UCNPs. There was a good linear relationship between the luminescence recovery intensity of UCNPs and GST concentration, ranging from 0.11 to 14.19 nM, and the detection of limit was 0.06 nM. The method has been used in th...Continue Reading

References

Nov 4, 2006·Chemical Communications : Chem Comm·Wenhui ZhouKeith V Wood
Jun 15, 2013·Journal of the American Chemical Society·Yi LiuFuyou Li
Jul 26, 2013·Bioconjugate Chemistry·Alden E Voelker, Rajesh Viswanathan
Mar 13, 2014·ACS Applied Materials & Interfaces·Xiaojuan NiuLingxin Chen
Dec 17, 2016·Accounts of Chemical Research·Qianqian SuFuyou Li
Feb 18, 2017·ACS Applied Materials & Interfaces·Jianming ZhangJerome P Claverie
Apr 13, 2018·ACS Nano·Freddy T RabouwAndries Meijerink
Sep 28, 2018·Analytical Chemistry·Zhiming ZhangYong Zhang
Mar 15, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Elina AndresenMichael Schäferling

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Citations

Aug 20, 2021·Chemistry : a European Journal·Teng ZhangYasuchika Hasegawa

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