Turn-on fluorescent sensing of glutathione S-transferase at near-infrared region based on FRET between gold nanoclusters and gold nanorods

ACS Applied Materials & Interfaces
Long QinYukui Zhang

Abstract

A fluorescence resonance energy transfer (FRET) method based on gold nanoclusters capped glutathione (AuNCs@GSH) and amine-terminated gold nanorods (AuNRs) is designed for turn-on and near-infrared region (NIR) sensing of glutathione S-transferase (GST). The absorption band of AuNRs is tuned carefully to maximize the spectra overlap and enhance the efficiency of FRET. The FRET from multiple AuNCs to single AuNR quenches about 70% fluorescence emission of AuNCs. After GST is added, the strong specific interaction of GSH-GST can replace the AuNCs@GSH from AuNRs, FRET based on electrostatic interaction between AuNCs@GSH and AuNRs is switched off. Thus, emission enhancement of AuNCs@GSH is observed. The fluorescent enhancement is linearly with the increasing GST concentration over the range of 2-100 nM GST and the limit of detection for GST is about 1.5 nM.

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Citations

Feb 20, 2016·Biosensors & Bioelectronics·Jung Eun KimHyukjin Lee
Feb 26, 2016·ACS Applied Materials & Interfaces·Lingyang ZhangXuanyong Liu
May 6, 2016·The Analyst·Xiao-Rong SongJianping Xie
Sep 25, 2017·Critical Reviews in Biotechnology·Fereniki PerperopoulouNikolaos E Labrou
Jun 6, 2019·Chemical Communications : Chem Comm·Aiguo SongHui Chen
Jun 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Youkun ZhengXuemei Wang
Oct 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sebastián A DíazEunkeu Oh
Aug 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi HeXueji Zhang
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bing ChenFeng Wang
Jul 25, 2020·Analytical and Bioanalytical Chemistry·Hong ZhangZhaopeng Song
Dec 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Puneet Khandelwal, Pankaj Poddar
Sep 22, 2020·Nano Letters·Ryan M WilliamsDaniel A Heller

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