Turn-On Fluoresence Sensor for Hg2+ in Food Based on FRET between Aptamers-Functionalized Upconversion Nanoparticles and Gold Nanoparticles

Journal of Agricultural and Food Chemistry
Yan LiuQuansheng Chen

Abstract

In this study, a turn-on nanosensor for detecting Hg2+ was developed based on the fluorescence resonance energy transfer (FRET) between long-strand aptamers-functionalized upconversion nanoparticles (UCNPs) and short-strand aptamers-functionalized gold nanoparticles (GNPs). In the absence of Hg2+, FRET between UCNPs and GNPs occurred because of the specific matching between two aptamers, resulting in the fluorescence quenching of UCNPs. In the presence of Hg2+, long-stranded aptamers fold back into a hairpin structure due to the stable binding interactions between Hg2+ and thymine, leading to the release of GNPs from UCNPs, resulting in the quenched fluorescence restoration. Under the optimized conditions, the nanosensor achieved a linear detection range of 0.2-20 μM and a low detection limit (LOD) of 60 nM. Meanwhile, it showed good selectivity and has been applied to detecting Hg2+ in tap water and milk samples with good precision.

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Citations

Feb 3, 2019·Sensors·Nerea De AchaFrancisco J Arregui
Jul 25, 2019·Mikrochimica Acta·Zeki Semih PehlivanMeral Yüce
Nov 19, 2020·The Analyst·Riikka PeltomaaMaría C Moreno-Bondi
Mar 11, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fuyan XiaoGuofan Jin
Mar 14, 2021·Analytical and Bioanalytical Chemistry·Yuling WangGuofan Jin
Jun 3, 2021·Comprehensive Reviews in Food Science and Food Safety·Yawen RongQuansheng Chen
Jul 17, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yunlian ZhangQuansheng Chen
Jul 21, 2021·Critical Reviews in Food Science and Nutrition·Yi XuQuansheng Chen
Mar 27, 2019·Journal of Agricultural and Food Chemistry·Zhan ZhouQianming Wang

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