Turn-on theranostic fluorescent nanoprobe by electrostatic self-assembly of carbon dots with doxorubicin for targeted cancer cell imaging, in vivo hyaluronidase analysis, and targeted drug delivery

Biosensors & Bioelectronics
Na GaoXiaoling Zhang

Abstract

This paper reports a turn-on theranostic fluorescent nanoprobe P-CDs/HA-Dox obtained by electrostatic assembly of polyethylenimine (PEI)-modified carbon dots (P-CDs) and Hyaluronic acid (HA)-conjugated doxorubicin (Dox) for hyaluronidase (HAase) detection, self-targeted imaging and drug delivery. P-CDs/HA-Dox show weak emission in a physiological environment. By utilizing the high affinity of HA to CD44 receptors overexpressed on many cancer cells, P-CDs/HA-Dox are capable of targeting and penetrating into cancer cells, where they are activated by HAase. As a result, HA-Dox can be digested into small fragments, causing the release of Dox and thereby restoring the fluorescence of P-CDs. The theranostic fluorescent nanoprobe can effectively distinguish cancer cells from normal cells. The as-prepared nanoprobe achieves a sensitive assay of HAase with a detection limit of 0.65UmL-1. Furthermore, upon Dox release, the Dox could efficiently induce apoptosis in HeLa cells, as confirmed by MTT assay. The design of such a turn-on theranostic fluorescent probe provides a new strategy for self-targeted and image-guided chemotherapy.

Citations

May 24, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Yao LongManli Guo
Jul 2, 2019·Current Pharmaceutical Design·Baskar ThangarajSrinivasan Ranganathan
Aug 21, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Wei LiuHanbing Rao
Oct 21, 2019·Biomedical Chromatography : BMC·Amir ShakouriJalal Abdolalizadeh
Aug 8, 2019·Expert Opinion on Drug Delivery·Yu Sakurai, Hideyoshi Harashima
Aug 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuman DongPeng Liu
Mar 10, 2019·Mikrochimica Acta·Meghdad PirsahebMehrdad Payandeh
Feb 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zihnil Adha Islamy MazradSung Young Park
Nov 25, 2017·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Hui Feng, Zhaosheng Qian
Jan 4, 2019·Analytical and Bioanalytical Chemistry·Qianqian DuanShengbo Sang
Dec 20, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Xiaoke WangYan Zhao
Nov 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lizhen ZhangXing-Can Shen
Dec 11, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Xiaoliang HaoZhigang Fang
Nov 22, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mara GonçalvesHelena Tomás
Sep 24, 2020·Current Topics in Medicinal Chemistry·Menghan GaoWeiqi Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.