Turning up the heat: heat stress induces markers of programmed cell death in Plasmodium falciparum in vitro

Cell Death & Disease
Dewaldt Engelbrecht, Thérèsa Louise Coetzer

Abstract

Malaria is characterised by cyclical febrile episodes that result from the rupture of mature schizont-infected erythrocytes releasing merozoites. In patients infected with Plasmodium falciparum, fever may reach peak temperatures as high as 41 °C. Febrile episodes typically have a deleterious effect on parasites and probably benefit the host by aiding parasite clearance; however, the parasite may also gain advantage from limiting its burden on the host and prolonging infection to ensure development and transmission of slow-maturing gametocytes. Programmed cell death (PCD) may provide the parasite with a mechanism of self-limitation, although the occurrence and phenotype of PCD in the erythrocytic stages remain controversial due to conflicting data. This study aimed to characterise the cell death phenotype of P. falciparum in response to in vitro heat stress. A variety of biochemical markers of PCD, including DNA fragmentation, mitochondrial dysregulation and phosphatidylserine externalisation, as well as morphological studies of Giemsa-stained thin smears and real-time microscopy were utilised to characterise the phenotype. Heat stress decreased P. falciparum growth and development in vitro. Late-stage parasites were more suscep...Continue Reading

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Citations

Jan 23, 2016·Malaria Journal·Warren Antonio Vieira, Thérèsa L Coetzer
Oct 1, 2015·Malaria Journal·Dewaldt Engelbrecht, Thérèsa Louise Coetzer
Jul 9, 2016·Methods : a Companion to Methods in Enzymology·Wan Ni ChiaKevin Shyong-Wei Tan
Jul 19, 2016·Parasitology International·Dewaldt Engelbrecht, Thérèsa Louise Coetzer
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Mar 14, 2021·Cellular and Molecular Life Sciences : CMLS·Merryn FraserAlexander G Maier

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Methods Mentioned

BETA
flow
fluorescence microscopy
flow cytometry
dot blot

Software Mentioned

GraphPad Prism

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