Turnip Mosaic Virus Uses the SNARE Protein VTI11 in an Unconventional Route for Replication Vesicle Trafficking

The Plant Cell
Daniel Garcia CabanillasJean-François Laliberté

Abstract

Infection of plant cells by RNA viruses leads to the generation of organelle-like subcellular structures that contain the viral replication complex. During Turnip mosaic virus (TuMV) infection of Nicotiana benthamiana, the viral membrane protein 6K2 plays a key role in the release of motile replication vesicles from the host endoplasmic reticulum (ER). Here, we demonstrate that 6K2 contains a GxxxG motif within its predicted transmembrane domain that is vital for TuMV infection. Replacement of the Gly with Val within this motif inhibited virus production, and this was due to a relocation of the viral protein to the Golgi apparatus and the plasma membrane. This indicated that passage of 6K2 through the Golgi apparatus is a dead-end avenue for virus infection. Impairing the fusion of transport vesicles between the ER and the Golgi apparatus by overexpression of the SNARE Sec22 protein resulted in enhanced intercellular virus movement. Likewise, expression of nonfunctional, Golgi-located synaptotagmin during infection enhanced TuMV intercellular movement. 6K2 copurified with VTI11, a prevacuolar compartment SNARE protein. An Arabidopsis thaliana vti11 mutant was completely resistant to TuMV infection. We conclude that TuMV replica...Continue Reading

Citations

Apr 4, 2019·Molecular Plant Pathology·Annette Niehl, Manfred Heinlein
Jun 24, 2019·The New Phytologist·Liang ZhangJinxing Lin
Aug 28, 2019·Molecular Plant-microbe Interactions : MPMI·Bernardo RodamilansJuan Antonio García
Sep 29, 2019·The Plant Journal : for Cell and Molecular Biology·Guanwei WuAiming Wang
Aug 11, 2019·International Journal of Molecular Sciences·Hai ZhangJingsheng Xu
May 16, 2020·Frontiers in Plant Science·Katarzyna Otulak-KoziełRodrigo A Valverde
Mar 29, 2019·Genome Biology and Evolution·Rubén GonzálezSantiago F Elena
Feb 12, 2021·Plant Science : an International Journal of Experimental Plant Biology·Chaofan ChenXu Chen
Nov 3, 2020·AIMS Microbiology·Sergey Y Morozov, Andrey G Solovyev
Aug 5, 2021·Scientific Reports·Ricardo José Gonzaga PimentaAnete Pereira de Souza

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