Turnover of histones and histone variants in postnatal rat brain: effects of alcohol exposure

Clinical Epigenetics
Nadia RachdaouiDipak Sarkar

Abstract

Alcohol consumption during pregnancy is a significant public health problem and can result in a continuum of adverse outcomes to the fetus known as fetal alcohol spectrum disorders (FASD). Subjects with FASD show significant neurological deficits, ranging from microencephaly, neurobehavioral, and mental health problems to poor social adjustment and stress tolerance. Neurons are particularly sensitive to alcohol exposure. The neurotoxic action of alcohol, i.e., through ROS production, induces DNA damage and neuronal cell death by apoptosis. In addition, epigenetics, including DNA methylation, histone posttranslational modifications (PTMs), and non-coding RNA, play an important role in the neuropathology of FASD. However, little is known about the temporal dynamics and kinetics of histones and their PTMs in FASD. We examined the effects of postnatal alcohol exposure (PAE), an animal model of human third-trimester equivalent, on the kinetics of various histone proteins in two distinct brain regions, the frontal cortex, and the hypothalamus, using in vivo 2H2O-labeling combined with mass spectrometry-based proteomics. We show that histones have long half-lives that are in the order of days. We also show that H3.3 and H2Az histone v...Continue Reading

References

Jun 1, 1991·Alcoholism, Clinical and Experimental Research·A J GarroC S Lieber
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S L CommerfordE P Cronkite
Mar 1, 1996·Journal of Mass Spectrometry : JMS·C A FernandezH Brunengraber
Jun 12, 1998·Brain Research. Brain Research Reviews·J Luo, M W Miller
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BednarC L Woodcock
Jun 29, 2000·Neurochemistry International·P J Brooks
Apr 17, 2001·Apoptosis : an International Journal on Programmed Cell Death·J W OlneyC Ikonomidou
Apr 6, 2002·Science·Arkady CelesteAndré Nussenzweig
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Craig H BassingFrederick W Alt
Sep 25, 2002·Neurobiology of Disease·Krista L MoulderSteven Mennerick
Jan 11, 2003·Neurotoxicology·John W Olney
Nov 25, 2003·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·F LamarcheL Barret
Apr 16, 2004·Cerebral Cortex·Julie A Siegenthaler, Michael W Miller
Jun 16, 2005·Experimental Biology and Medicine·Edward P Riley, Christie L McGee
Jun 5, 2007·Nature Protocols·David ShechterSandra B Hake
Feb 20, 2008·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Bruce AnthonyJoseph Ruiz
Oct 18, 2008·Nature Reviews. Genetics·William F MarzluffRobert J Duronio
Jan 8, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Peter G WellsAndrea W Wong
May 22, 2009·Biology of Reproduction·Philip C Haycock
Jul 4, 2009·Alcoholism, Clinical and Experimental Research·Susan J AstleyTodd Richards
Jul 7, 2009·Nature Cell Biology·Rakesh Kumar SinghAkash Gunjan
Sep 3, 2009·Molecular & Cellular Proteomics : MCP·Nadia RachdaouiStephen F Previs
Sep 5, 2009·Developmental Disabilities Research Reviews·Andria L NormanEdward P Riley
May 22, 2010·Analytical Biochemistry·Vinit ShahThomas P Roddy
Dec 8, 2010·Epigenetics & Chromatin·Barry M ZeeBenjamin A Garcia
Jul 4, 2012·Molecular Cell·Gaston SoriaGeneviève Almouzni
Jul 25, 2012·Alcoholism, Clinical and Experimental Research·Nadka I Boyadjieva, Dipak K Sarkar

❮ Previous
Next ❯

Citations

Jan 4, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Taylor L GretzingerJuan Ausió
Jan 6, 2018·Neurotoxicity Research·Yousef TizabiRobert E Taylor
Feb 28, 2018·Frontiers in Immunology·Miriam G Jasiulionis
Dec 20, 2020·Analytical Biochemistry·Andrea Arias-AlvaradoTakhar Kasumov

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
ELISA
fluorescence microscopy

Software Mentioned

Sequest
ImageJ
ITCN ( Image - based Tool for Counting Nuclei for ImageJ
Thermo Proteome Discoverer

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved