PMID: 1204651Dec 15, 1975Paper

Turnover of polyadenylated messenger RNA in fission yeast. Evidence for the control of protein synthesis at the translational level

European Journal of Biochemistry
R S Fraser

Abstract

Polyadenylated RNA was isolated from fission yeast (Schizosaccharomyces pombe) total RNA using oligo(dT)-cellulose, and was studied as a model for messenger RNA. The half-life of poly adenylated RNA was measured by two independent methods. (a) The rate of labelling of polyadenylated RNA during incubation of cells with [5-3H]uridine was measured. A half-life of 40-45 min was found by comparing the experimental data with theoretical curves calculated for labelling of RNAs with various half-lives. The influence of precursor-pool specific activity on RNA labelling kinetics is considered. (b) Cells were labelled with [5-3H]uridine then further RNA synthesis was inhibited by addition of 8-hydroxyquinoline. The rate of loos of radioactivity from polyadenylated RNA indicated a half-life of 50 min. The half-life found by these two methods is about one-third of the cell doubling time, and is much longer than previous estimates by indirect methods of yeast messenger RNA half-life. Both experimental methods provided evidence for the existence of tas a half-life of 40-50 min; a much smaller population is probably turning over more rapidly. After inhibition of RNA synthesis by 8-hydroxyquinoline, the rate of total protein synthesis declined ...Continue Reading

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Citations

Oct 4, 1978·Molecular & General Genetics : MGG·J C BlochF Lacroute
May 1, 1984·Current Genetics·F Pelsy, F Lacroute
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R Losson, F Lacroute
Dec 15, 1975·European Journal of Biochemistry·J CreanorJ M Mitchison
Oct 26, 2010·Current Biology : CB·Jacob ZhurinskyPaul Nurse
Nov 18, 2006·Cell·Cintia F HongayGerald R Fink
May 10, 2017·Journal of Cell Science·Louise WestonPaul Nurse
Mar 1, 1981·Microbiological Reviews·L Alberghina, E Sturani
Jul 1, 1977·Journal of Bacteriology·J Bossinger, T G Cooper
Jun 14, 1976·Journal of Molecular Biology·R S Fraser, B L Carter

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