Twin-column CaptureSMB: a novel cyclic process for protein A affinity chromatography

Journal of Chromatography. a
Monica AngaritaM Morbidelli

Abstract

A twin-column counter-current chromatography processes, CaptureSMB, was used for the protein A affinity capture of a monoclonal antibody (mAb). By means of sequential loading, the process improves the utilization of the stationary phase by achieving loadings much closer to the static binding capacity of the resin in comparison to batch chromatography. Using a mAb capture case study with protein A affinity chromatography, the performance and product quality obtained from CaptureSMB and batch processes were compared. The effect of the flow rate, column length and titer concentration on the process performance and product quality were evaluated. CaptureSMB showed superior performance compared to batch chromatography with respect to productivity, capacity utilization, product concentration and buffer consumption. A simplified economic evaluation showed that CaptureSMB could decrease resin costs of 10-30% depending on the manufacturing scenario.

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Citations

Jan 27, 2016·Journal of Chromatography. a·Carlos Andrés Martínez Cristancho, Andreas Seidel-Morgenstern
Jul 15, 2015·Biotechnology and Bioengineering·Andrew L Zydney
Apr 6, 2016·Journal of Chromatography. a·Fabian SteinebachMassimo Morbidelli
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Jul 23, 2016·Journal of Chromatography. a·Rafał GorczycaDorota Antos
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Aug 30, 2016·Computational and Structural Biotechnology Journal·Renaud JacquemartJim Stout
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Oct 10, 2018·Biotechnology and Bioengineering·Daniel BaurMassimo Morbidelli
Jul 12, 2017·Biotechnology Progress·Fabian SteinebachMassimo Morbidelli
May 8, 2019·Biotechnology and Bioengineering·James AngeloZhengjian Li
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