Twist expression associated with the epithelial-mesenchymal transition in gastric cancer.

Molecular and Cellular Biochemistry
Ai-ning LiuXiao-sheng Hang

Abstract

This study aimed to investigate the expression of Twist in gastric cancer tissues and its correlation between Twist and the epithelial-mesenchymal transition (EMT). By means of RT-PCR and Western blot, the mRNA and protein expressions of Twist, E-cadherin, and Vimentin in 61 gastric cancer tissues and adjacent normal tissues were detected. The positive rates of Twist, E-cadherin, and Vimentin mRNA expression in gastric cancer tissues were 73.9. 40.6, and 60.9 %, respectively; compared to the expression of these genes in adjacent normal tissues (2.9, 75.4, and 27.5 %), the differences were significant (p < 0.05). The E-cadherin protein expression level in gastric cancer tissues was significantly lower than that in the adjacent normal tissues (p < 0.05). After the transfection of Twist siRNA into the MKN45 cells, the protein expression of Twist was significantly reduced (p < 0.05), the protein expression of E-cadherin was significantly increased, and the number of cells that passed through the Transwell chamber was significantly lower than that in the non-transfected control group as well as the transfected control group (p < 0.05). Twist may be associated with the epithelial-mesenchymal transition in gastric cancer and the tumor...Continue Reading

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Citations

Jan 31, 2015·Molecular Medicine Reports·Jianwei ZhuXiancheng Liu
Aug 13, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ping XiaoRuirui Yang
Dec 15, 2015·Pharmacognosy Magazine·Hyun Soo ParkByung Joo Kim
Oct 8, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hai-Ting Liu, Peng Gao
May 2, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Pu Xia, Xiao-Yan Xu
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Apr 10, 2020·International Journal of Molecular Sciences·Jacek BajPiero Portincasa

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