PMID: 25744835Mar 7, 2015Paper

Twist gene promotes the formation and migration of mammospheres of BT-549 breast cancer cells

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Mingli ZhouManran Liu

Abstract

To investigate the effect of Twist gene deficiency on the enrichment and migration of breast cancer stem-like cells (CSCs). Twist gene in the BT-549 cell line was knocked down by shRNA interference technology, and the cell line BT-549-shVec was used as the negative control group. Interference efficiency was determined by real-time quantitative RT-PCR (qRT-PCR) and Western blotting. Mammospheres with the characteristics of stem-like cells were collected by long-term serum-free suspension cultivation. The migration ability of mammospheres was analyzed by Transwell(TM) assay. The BT-549-shTwist breast cancer cells were successfully established by shRNA interference technology. The enrichment and migration abilities of CSCs from the BT-549-shTwist cell line decreased significantly. Twist gene promotes the enrichment and migration of CSCs from the BT-549 cell line.

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