Twist promotes tumor metastasis in basal-like breast cancer by transcriptionally upregulating ROR1.

Theranostics
Jingying CaoTie-Bang Kang

Abstract

Rationale: Twist is a key transcription factor for induction of epithelial-mesenchymal transition (EMT), which promotes cell migration, invasion, and cancer metastasis, confers cancer cells with stem cell-like characteristics, and provides therapeutic resistance. However, the functional roles and targeted genes of Twist in EMT and cancer progression remain elusive. Methods: The potential targeted genes of Twist were identified from the global transcriptomes of T47D/Twist cells by microarray analysis. EMT phenotype was detected by western blotting and immunofluorescence of marker proteins. The dual-luciferase reporter and chromatin immunoprecipitation assays were employed to observe the direct transcriptional induction of ROR1 by Twist. A lung metastasis model was used to study the pro-metastatic role of Twist and ROR1 by injecting MDA-MB-231 cells into tail vein of nude mice. Bio-informatics analysis was utilized to measure the metastasis-free survival of breast cancer patients. Results: Twist protein was proved to directly activate the transcription of ROR1 gene, a receptor of Wnt5a in non-canonical WNT signaling pathway. Silencing of ROR1 inhibited EMT process, cell migration, invasion, and cancer metastasis of basal-like bre...Continue Reading

Citations

Dec 13, 2019·Journal of Receptor and Signal Transduction Research·Yunpeng TianHaoming Song
Dec 21, 2019·Journal of Receptor and Signal Transduction Research·Yang YangZhiyun Wang
Jan 29, 2020·Journal of Receptor and Signal Transduction Research·Peng LiQiuyun Tu
Jul 2, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Q GuoJ Lin
Nov 23, 2019·Journal of Receptor and Signal Transduction Research·Guanqun Ma, Yingwu Liu
Feb 23, 2020·Frontiers in Cell and Developmental Biology·Emma H van Schie, Renée van Amerongen
Mar 31, 2019·Cell Death & Disease·Dong HanXiaoyuan Chu
Oct 9, 2020·Signal Transduction and Targeted Therapy·Siyuan QinWeifeng He
Jan 16, 2021·Cells·Kerstin MenckAnnalen Bleckmann
Jan 6, 2021·Molecular Cancer·Xiaoxu WeiZhaoyang Zeng
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Jiaxin LiuGang Yin
Jul 30, 2021·Breast Cancer Research and Treatment·Ann Hanna, Justin M Balko
Aug 8, 2021·International Journal of Molecular Sciences·Shailendra Pratap SinghAbhinav Kanwal

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE53222

Methods Mentioned

BETA
Assay
immunoprecipitation
ChIP

Software Mentioned

SPSS
Affymetrix Expression Console

Related Concepts

Related Feeds

Breast Cancer Triple-N

Breast cancer cells have receptors for estrogen, progesterone, HER2 receptors (also called ERBB2). Triple-negative breast cancers do not have any of these receptors. Here are the latest discoveries pertaining to triple-negative breast cancers.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Related Papers

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xiaoting HuangJide Wang
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Jing WangJide Wang
© 2022 Meta ULC. All rights reserved