Twitching and Swimming Motility Play a Role in Ralstonia solanacearum Pathogenicity.

MSphere
Jordi CorralMarc Valls

Abstract

Ralstonia solanacearum is a bacterial plant pathogen causing important economic losses worldwide. In addition to the polar flagella responsible for swimming motility, this pathogen produces type IV pili (TFP) that govern twitching motility, a flagellum-independent movement on solid surfaces. The implication of chemotaxis in plant colonization, through the control flagellar rotation by the proteins CheW and CheA, has been previously reported in R. solanacearum In this work, we have identified in this bacterium homologues of the Pseudomonas aeruginosa pilI and chpA genes, suggested to play roles in TFP-associated motility analogous to those played by the cheW and cheA genes, respectively. We demonstrate that R. solanacearum strains with a deletion of the pilI or the chpA coding region show normal swimming and chemotaxis but altered biofilm formation and reduced twitching motility, transformation efficiency, and root attachment. Furthermore, these mutants displayed wild-type growth in planta and impaired virulence on tomato plants after soil-drench inoculations but not when directly applied to the xylem. Comparison with deletion mutants for pilA and fliC-encoding the major pilin and flagellin subunits, respectively-showed that bot...Continue Reading

References

Dec 24, 1997·Applied and Environmental Microbiology·F BertollaP Simonet
May 24, 2001·Journal of Bacteriology·J Tans-KerstenC Allen
Jul 27, 2002·Annual Review of Microbiology·John S Mattick
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
Jul 1, 1984·Applied and Environmental Microbiology·C A Hendrick, L Sequeira
Jan 24, 2007·Molecular Microbiology·Robert P RyanJ Maxwell Dow
Nov 21, 2008·Molecular Plant Pathology·Yvonne McCarthyJ Maxwell Dow
Jul 11, 2009·Molecular Plant-microbe Interactions : MPMI·Ofir BaharSaul Burdman
Mar 23, 2011·Journal of Bacteriology·Fanhong MengCaitilyn Allen
May 11, 2011·Advances in Experimental Medicine and Biology·Nadia Mhedbi-HajriRalf Koebnik
Jun 23, 2011·Molecular Plant-microbe Interactions : MPMI·Luciana CursinoPatricia Mowery
Jun 8, 2012·Molecular Plant Pathology·John MansfieldGary D Foster
May 31, 2013·Molecular Plant Pathology·Nemo PeetersMarc Valls
Dec 4, 2013·Nucleic Acids Research·Sean PowellPeer Bork
Mar 15, 2014·Molecular Plant-microbe Interactions : MPMI·María Inés SiriMaría Julia Pianzzola
Jan 1, 2013·Biology·Johanna Haiko, Benita Westerlund-Wikström
Jun 5, 2014·Trends in Microbiology·Robert Belas
Aug 8, 2014·FEMS Microbiology Reviews·Inmaculada SampedroJane E Hill
Jan 1, 2016·Biochemical and Biophysical Research Communications·Erlia NarulitaTakashi Yamada
May 12, 2016·Journal of Visualized Experiments : JoVE·Xiangyang Shi, Hong Lin
Jan 24, 2017·Phytopathology·Denise CaldwellAnjali S Iyer-Pascuzzi
Dec 31, 2017·Methods in Molecular Biology·Arry MorelAlice Guidot

❮ Previous
Next ❯

Citations

Nov 17, 2020·Frontiers in Microbiology·Sivasamy SethupathyJintae Lee
Mar 9, 2021·Current Opinion in Microbiology·Vadim M GumerovIgor B Zhulin
Feb 23, 2021·Microbiological Research·Hing Jian MeaEng Hwa Wong
Jul 7, 2021·FEMS Microbiology Reviews·Remy ColinVictor Sourjik
Aug 10, 2021·Frontiers in Plant Science·Jeffrey K Schachterle, Qi Huang

❮ Previous
Next ❯

Datasets Mentioned

BETA
GMI1000

Methods Mentioned

BETA
PCR
reverse transcription-PCRs
light microscopy
PCRs

Software Mentioned

PilS
eggNOG
PilR

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Related Papers

Annual Review of Microbiology
J Henrichsen
Annual Review of Microbiology
J S Mattick
FEMS Microbiology Letters
David A Coil, Jozef Anné
© 2021 Meta ULC. All rights reserved