Two AAA family peroxins, PpPex1p and PpPex6p, interact with each other in an ATP-dependent manner and are associated with different subcellular membranous structures distinct from peroxisomes.

Molecular and Cellular Biology
K N FaberS Subramani

Abstract

Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris. Cells from the corresponding deletion strains (Pp delta pex1 and Pp delta pex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the cytosol, and these cells cannot grow in peroxisome-requiring media (J. A. Heyman, E. Monosov, and S. Subramani, J. Cell Biol. 127:1259-1273, 1994; A. P. Spong and S. Subramani, J. Cell Biol. 123:535-548, 1993). We demonstrate that PpPex1p and PpPex6p interact in an ATP-dependent manner. Genetically, the interaction was observed in a suppressor screen with a strain harboring a temperature-sensitive allele of PpPEX1 and in the yeast two-hybrid system. Biochemially, these proteins were coimmunoprecipitated with antibodies raised against either of the proteins, but only in the presence of ATP. The protein complex formed under these conditions was 320 to 400 kDa in size, consistent with the formation of a heterodimeric PpPex1p-PpPex6p complex. Subcellular fractionation revealed PpPex1p and PpPex6p to be predominantly associated with membranous subcellular structures distinct from peroxisomes. Based on their behavior in ...Continue Reading

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Citations

Feb 3, 2016·The Journal of Cell Biology·Gaurav AgrawalSuresh Subramani
Feb 5, 2016·Scientific Reports·Immanuel GrimmRalf Erdmann
Jul 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Neil B BlokThomas Walz
Jan 7, 2005·The FEBS Journal·Ingvild BirschmannWolf-H Kunau
Feb 11, 2015·Journal of Molecular Biology·Brooke M GardnerAndreas Martin
Aug 9, 2005·Human Mutation·Denis I CraneBarbara C Paton
Feb 1, 2005·Clinical Genetics·R J A Wanders, H R Waterham
May 21, 2009·IUBMB Life·Changle Ma, Suresh Subramani
Oct 11, 2015·Biochimica Et Biophysica Acta·Immanuel GrimmWolfgang Girzalsky
May 26, 2006·Biochemical and Biophysical Research Communications·Wei Fan, Yukio Fujiki
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Feb 18, 2003·Molecular Biology of the Cell·Paulina OzimekMarten Veenhuis
Feb 21, 2002·Molecular Biology of the Cell·Noriyo HashiguchiTakashi Osumi
Jul 3, 2009·Molecular Biology of the Cell·Changle MaSuresh Subramani

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