Two adenine nucleotide translocase paralogues involved in cell proliferation and spermatogenesis in the silkworm Bombyx mori

PloS One
Ryohei SugaharaTakahiro Shiotsuki

Abstract

Mitochondrial adenine nucleotide translocase (ANT) specifically acts in ADP/ATP exchange through the mitochondrial inner membrane. This transporter protein thereby plays a significant role in energy metabolism in eukaryotic cells. Most mammals have four paralogous ANT genes (ANT1-4) and utilize these paralogues in different types of cells. The fourth paralogue of ANT (ANT4) is present only in mammals and reptiles and is exclusively expressed in testicular germ cells where it is required for meiotic progression in the spermatocytes. Here, we report that silkworms harbor two ANT paralogues, the homeostatic paralogue (BmANTI1) and the testis-specific paralogue (BmANTI2). The BmANTI2 protein has an N-terminal extension in which the positions of lysine residues in the amino acid sequence are distributed as in human ANT4. An expression analysis showed that BmANTI2 transcripts were restricted to the testis, suggesting the protein has a role in the progression of spermatogenesis. By contrast, BmANTI1 was expressed in all tissues tested, suggesting it has an important role in homeostasis. We also observed that cultured silkworm cells required BmANTI1 for proliferation. The ANTI1 protein of the lepidopteran Plutella xylostella (PxANTI1),...Continue Reading

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Citations

Feb 2, 2016·Journal of Insect Physiology·Minoru TanigawaYoko Nagata
Aug 23, 2018·Histochemistry and Cell Biology·Tomohide UnoAkira Mizoguchi
Apr 5, 2019·Archives of Insect Biochemistry and Physiology·Ryohei Sugahara, Seiji Tanaka
May 20, 2016·Journal of Pesticide Science·Ryohei SugaharaTakahiro Shiotsuki

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Datasets Mentioned

BETA
DRA002231

Methods Mentioned

BETA
RNA-seq
reverse transcription-PCR
transfection
in vitro transcription
PCR

Software Mentioned

Ensembl
GENETYX
TOSSGAPS
VENUS
IMAGEJ
CLUSTAL

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