Two adjacent docking sites in the yeast Hog1 mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase.

Molecular and Cellular Biology
Yulia MurakamiHaruo Saito

Abstract

Functional interactions between a mitogen-activated protein kinase (MAPK) and its regulators require specific docking interactions. Here, we investigated the mechanism by which the yeast osmoregulatory Hog1 MAPK specifically interacts with its activator, the MAPK kinase Pbs2, and its major inactivator, the protein phosphatase Ptp2. We found, in the N-terminal noncatalytic region of Pbs2, a specific Hog1-binding domain, termed HBD-1. We also defined two adjacent Pbs2-binding sites in Hog1, namely, the common docking (CD) domain and Pbs2-binding domain 2 (PBD-2). The PBD-2 docking site appears to be sterically blocked in the intact Hog1 molecule, but its affinity to Pbs2 is apparent in shorter fragments of Hog1. Both the CD and the PBD-2 docking sites are required for the optimal activation of Hog1 by Pbs2, and in the absence of both sites, Hog1 cannot be activated by Pbs2. These data suggest that the initial interaction of Pbs2 with the CD site might induce a conformational change in Hog1 so that the PBD-2 site becomes accessible. The CD and PBD-2 docking sites are also involved in the specific interaction between Hog1 and Ptp2 and govern the dynamic dephosphorylation of activated Hog1. Thus, the CD and the PBD-2 docking sites p...Continue Reading

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Citations

Jan 1, 2009·Médecine sciences : M/S·Pascal HersenSharad Ramanathan
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Feb 27, 2014·Science Signaling·Keiichiro TanakaHaruo Saito
Apr 26, 2012·Molecular Systems Biology·Carl-Fredrik TigerMarcus Krantz
Oct 1, 2010·Current Opinion in Microbiology·Haruo Saito
Apr 1, 2015·FEMS Microbiology Letters·Anja KejžarMetka Lenassi
Dec 9, 2014·Fungal Genetics and Biology : FG & B·Anja KejžarMetka Lenassi
May 31, 2018·Molecular Biology of the Cell·Jongmin Lee, David E Levin
Apr 22, 2015·Nature Communications·Kazuo TatebayashiHaruo Saito
Jun 22, 2020·Current Genetics·Araceli Vázquez-IbarraRoberto Coria
Dec 19, 2017·Journal of Molecular Modeling·Natalee Carapia-MineroAída Verónica Rodríguez-Tovar
Dec 1, 2017·Journal of Cell Science·Kosuke ShiraishiYasuyoshi Sakai
Nov 1, 2020·International Journal of Molecular Sciences·Sudip Dhakal, Ian Macreadie

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