PMID: 6404630Apr 15, 1983Paper

Two alpha-3-D-galactosyltransferases in rabbit stomach mucosa with different acceptor substrate specificities

European Journal of Biochemistry
A Betteridge, W M Watkins

Abstract

Homogenates of rabbit stomach mucosa were examined for enzymes catalysing the transfer of D-galactose from UDP-D-galactose to various low-molecular-weight acceptors of known structure. Treatment of the products with alpha and beta-D-galactosidases revealed that D-galactose was transferred in both alpha and beta-anomeric linkages. The beta-D-galactosyltransferase used N-acetylglucosamine and compounds containing terminal nonreducing beta-N-acetylglucosaminyl residues as acceptor substrates. The compounds accepting D-galactose in alpha-anomeric linkage had unsubstituted terminal non-reducing beta-D-galactosyl units or a fucose substituent on the carbon-2 position of a subterminal beta-D-galactosyl unit. Methylation analysis of the products formed with N-acetyllactosamine [beta-D-Galp(1 leads to 4)D-GlcNAcp] and 2'fucosyllactose [alpha-L-Fucp(1 leads to 2)-beta-D-Galp(1 leads to 4)D-Glcp] revealed that D-galactose was transferred to the carbon-3 position of the beta-D-galactosyl residue in both of these acceptor substrates. Competition experiments with the two substrates indicated that the transfer of D-galactose was catalysed in each case by a different alpha-3-D-galactosyltransferase. Differences were also observed in the solubi...Continue Reading

References

Oct 11, 1967·Biochemical and Biophysical Research Communications·D ZidermanW M Watkins
Jan 31, 1972·Biochemical and Biophysical Research Communications·V M HearnW M Watkins
May 7, 1968·Journal of Chromatography·W F Lehnhardt, R J Winzler
Jul 26, 1968·Biochemical and Biophysical Research Communications·A KobataV Ginsburg
Nov 1, 1981·European Journal of Biochemistry·A S Donald
Jun 26, 1981·Journal of Chromatography·A Anderson, A S Donald
Oct 16, 1970·FEBS Letters·Caroline Race, Winifred M. Watkins
Sep 1, 1971·FEBS Letters·T Sawicka
May 1, 1968·The Biochemical Journal·C RaceW M Watkins
Aug 30, 1973·Biochimica Et Biophysica Acta·A S Donald

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Citations

Jun 1, 1988·Transfusion Medicine Reviews·U Galili
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·U Galili, K Swanson
Aug 15, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Uri Galili

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