PMID: 2122453Oct 1, 1990Paper

Two amino acids in an RNA polymerase sigma factor involved in the recognition of adjacent base pairs in the -10 region of a cognate promoter

Proceedings of the National Academy of Sciences of the United States of America
D DanielsR Losick

Abstract

The recognition of promoter region -10 nucleotide sequences in prokaryotes is believed to be mediated by a segment of alpha-helix in a region of RNA polymerase sigma factors called 2.4. Earlier genetic studies implicated Thr-100 in region 2.4 of the Bacillus subtilis sigma factor sigma H in the recognition of the G.C base pair at position -13 in the -10 region (GAAT) of a cognate promoter. In confirmation of this assignment, we now show that a change-of-specificity mutant of sigma H in which Thr-100 was replaced with isoleucine suppresses a G.C----A.T nucleotide substitution at position -13 but not other "promoter down mutations" (causing impaired promoter activity) at positions -13, -12, and -11. We also show that a loss-of-contact mutant created by the replacement of Thr-100 with alanine (having a short side chain) enables sigma H to tolerate three different promoter down mutations at position -13 but not down mutations at other positions. Finally, we suggest the identification of an additional amino acid involved in base-pair recognition by the demonstration that the replacement of Arg-96 with alanine specifically suppresses an A.T----G.C promoter down mutation at position -12. The identification of amino acids that are four...Continue Reading

References

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Citations

Aug 5, 1991·Journal of Molecular Biology·J MecsasC A Gross
Nov 15, 1996·Structure·C L ChanC A Gross
Mar 4, 1999·Journal of Applied Microbiology·A FerreiraK J Boor
Nov 13, 2001·Molecular Microbiology·N E Baldwin, A J Dombroski
May 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·S SatolaC P Moran
Mar 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·C H Jones, C P Moran
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M OuhammouchE N Brody
Oct 17, 2009·Genes & Development·Andrey Feklistov, Seth A Darst
Sep 22, 2001·Protein Science : a Publication of the Protein Society·F D SchubotB C Wang
Oct 23, 2012·Science·Yu ZhangRichard H Ebright
Jun 30, 2000·Biochemical and Biophysical Research Communications·M J Wilson, I L Lamont
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·D M JooR Calendar
Apr 20, 1992·Journal of Molecular Biology·G Gonzy-TréboulP Stragier
May 31, 1991·Biochemical and Biophysical Research Communications·A Wellman, C F Meares
Oct 1, 1995·FEMS Microbiology Reviews·U SauerP Dürre
Apr 5, 2002·Molecular Cell·Elizabeth A CampbellSeth A Darst
Sep 28, 2002·The Journal of Biological Chemistry·Shun Jin Lee, Jay D Gralla
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·C A GrossB Young
Nov 18, 1998·FEMS Microbiology Reviews·M M Wösten
Nov 25, 2016·Frontiers in Molecular Biosciences·Jookyung Lee, Sergei Borukhov
Mar 20, 1999·The Journal of Biological Chemistry·O L Schaubach, A J Dombroski
Mar 27, 1997·Biochemical and Biophysical Research Communications·Y S TsengM T Yang

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