Two antigens on zygotes and ookinetes of Plasmodium yoelii and Plasmodium berghei that are distinct targets of transmission-blocking immunity.

Infection and Immunity
Takafumi TsuboiM Torii

Abstract

We have developed transmission-blocking monoclonal antibodies (MAbs) against Plasmodium yoelii 21-kDa (Pys21) and 28-kDa (Pys25) ookinete surface proteins. These MAbs block infectivity of P. yoelii to Anopheles stephensi. One MAb, 14, cross-reacted by Western blotting with a 28-kDa surface protein (Pbs25) of P. berghei ookinetes and blocked oocyst development, as assayed by direct mosquito feeds on passively immunized P. berghei-infected mice. In total, we have identified two ookinete surface proteins in P. yoelii, one of which is also present in P. berghei. The transmission-blocking activity of the anti-Pys25 MAb 4 was complete and more potent than that of the anti-Pys21 MAb 2. Moreover, Fab fragments of MAb 4 had transmission-blocking activity in mice. In comparison, Fab fragments of MAb 2 did not have detectable transmission-blocking effect, although F(ab')2 did. Furthermore, MAb 2 and MAb 4 appeared to block the in vitro formation and development of zygotes as well.

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Citations

Apr 11, 2008·The Journal of Biological Chemistry·Mathieu GissotKami Kim
Dec 6, 2008·Journal of Molecular Modeling·Babita SharmaAjay K Saxena
Jun 18, 2010·Clinical and Vaccine Immunology : CVI·Sung-Ung MoonHyeong-Woo Lee
Sep 16, 2000·Molecular and Biochemical Parasitology·M del Carmen RodriguezG Margos
May 4, 2021·Frontiers in Cellular and Infection Microbiology·Takaaki YuguchiHitoshi Otsuki
Mar 18, 1999·Molecular and Biochemical Parasitology·A R BlancoR E Sinden
Nov 9, 2000·Molecular and Biochemical Parasitology·D TaylorA Saul
Jan 25, 2003·Parasitology International·Takafumi TsuboiMotomi Torii
Oct 24, 2003·International Journal for Parasitology·Shin-ichiro KawazuShigeyuki Kano
Dec 8, 2004·International Journal for Parasitology·C K MoreiraM Jacobs-Lorena
Nov 18, 2005·Experimental Parasitology·Johanna M Porter-KelleyAbdu F Azad

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