Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation

Glycobiology
Hiroyuki KajiuraK Fujiyama

Abstract

N-Glycosylation is an important post-translational modification that occurs in many secreted and membrane proteins in eukaryotic cells. Golgi alpha-mannosidase I hydrolases (MANI) are key enzymes that play a role in the early N-glycan modification pathway in the Golgi apparatus. In Arabidopsis thaliana, two putative MANI genes, AtMANIa (At3g21160) and AtMANIb (At1g51590), were identified. Biochemical analysis using bacterially produced recombinant AtMANI isoforms revealed that both AtMANI isoforms encode 1-deoxymannojirimycin-sensitive alpha-mannosidase I and act on Man(8)GlcNAc(2) and Man(9)GlcNAc(2) structures to yield Man(5)GlcNAc(2). Structures of hydrolytic intermediates accumulated in the AtMANI reactions indicate that AtMANIs employ hydrolytic pathways distinct from those of mammalian MANIs. In planta, AtMANI-GFP/DsRed fusion proteins were detected in the Golgi stacks. Arabidopsis mutant lines manIa-1, manIa-2, manIb-1, and manIb-2 showed N-glycan profiles similar to that of wild type. On the other hand, the manIa manIb double mutant lines produced Man(8)GlcNAc(2) as the predominant N-glycan and lacked plant-specific complex and hybrid N-glycans. These data indicate that either AtMANIa or AtMANIb can function as the Golg...Continue Reading

References

Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·N Q PalacpacT Seki
Dec 2, 1999·Biochimica Et Biophysica Acta·A Herscovics
Dec 14, 1999·Plant Physiology·A NebenführL A Staehelin
Apr 15, 2000·Glycobiology·Z KawarD L Jarvis
Sep 21, 2000·The Journal of Biological Chemistry·F ValleeP L Howell
Mar 28, 2001·Science·A Helenius, M Aebi
Mar 20, 2002·The Journal of Cell Biology·C Stewart GillmorChris Somerville
Mar 7, 2003·Phytochemistry·Suzanne PaquetteSøren Bak
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·S PagnyV Gomord
Jun 11, 2004·Annual Review of Biochemistry·Robert S Haltiwanger, John B Lowe
Dec 1, 2005·Glycobiology·Daniel J Kelleher, Reid Gilmore
Jun 1, 1979·Plant Physiology·T Boller, H Kende
Nov 23, 2006·Methods in Enzymology·Steven W Mast, Kelley W Moremen
Apr 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jae Sook KangHisashi Koiwa
Aug 26, 2009·Plant Signaling & Behavior·Antje von SchaewenHisashi Koiwa

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Citations

Dec 17, 2009·The Journal of Biological Chemistry·Heidrun HäwekerAntje von Schaewen
Apr 27, 2012·Glycoconjugate Journal·Iain B H Wilson
Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Apr 1, 2011·Journal of Bioscience and Bioengineering·Junko Isoyama-TanakaKazuhito Fujiyama
Feb 6, 2015·Journal of Proteome Research·Ming ZhangYue-Ming Yan
Feb 26, 2016·Glycobiology·Richard Strasser
Sep 24, 2015·Plant Science : an International Journal of Experimental Plant Biology·Nausicaä Lannoo, Els J M Van Damme
Jun 23, 2018·Plant Signaling & Behavior·A von SchaewenH Koiwa
Nov 7, 2015·Nature Biotechnology·Robin PironNico Callewaert
May 1, 2020·Frontiers in Plant Science·Judit Sánchez-SimarroMaría Jesús Marcote
Feb 5, 2021·Frontiers in Plant Science·Makoto KatsubeYoshinobu Kimura
Aug 7, 2018·Plant Science : an International Journal of Experimental Plant Biology·Yukihiro NagashimaHisashi Koiwa
Mar 27, 2021·Frontiers in Plant Science·Heidi Kaulfürst-SobollAntje von Schaewen
Jun 3, 2021·Biomolecules·Tibo De ConinckEls J M Van Damme

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