PMID: 9636149Jun 24, 1998Paper

Two binding modes reveal flexibility in kinase/response regulator interactions in the bacterial chemotaxis pathway

Proceedings of the National Academy of Sciences of the United States of America
M M McEvoyF W Dahlquist

Abstract

The crystal structure at 2.0-A resolution of the complex of the Escherichia coli chemotaxis response regulator CheY and the phosphoacceptor-binding domain (P2) of the kinase CheA is presented. The binding interface involves the fourth and fifth helices and fifth beta-strand of CheY and both helices of P2. Surprisingly, the two heterodimers in the asymmetric unit have two different binding modes involving the same interface, suggesting some flexibility in the binding regions. Significant conformational changes have occurred in CheY compared with previously determined unbound structures. The active site of CheY is exposed by the binding of the kinase domain, possibly to enhance phosphotransfer from CheA to CheY. The conformational changes upon complex formation as well as the observation that there are two different binding modes suggest that the plasticity of CheY is an essential feature of response regulator function.

References

Jan 1, 1992·Annual Review of Genetics·J S Parkinson, E C Kofoid
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jan 1, 1985·Methods in Enzymology·A J HowardN H Xuong
Oct 1, 1995·Nature Structural Biology·R V SwansonF W Dahlquist
Oct 13, 1995·The Journal of Biological Chemistry·D Shukla, P Matsumura
Jan 5, 1993·Journal of Molecular Biology·G D Van DuyneJ Clardy
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·T B Morrison, J S Parkinson
Nov 9, 1993·Biochemistry·K Volz
Jan 1, 1995·Annual Review of Microbiology·D F Blair
Sep 20, 1997·The Journal of Biological Chemistry·X ZhuP Matsumura
Jan 23, 1998·Nature Structural Biology·M WelchJ P Samama
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

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Citations

Jan 27, 2004·Journal of Molecular Biology·Richard C Stewart, Ricaele Van Bruggen
Nov 8, 2002·Journal of Molecular Biology·Steven L Porter, Judith P Armitage
Dec 26, 2001·Current Opinion in Structural Biology·H S ChoD E Wemmer
Feb 5, 2000·Current Biology : CB·J Stock, M Levit
Jun 19, 2001·Trends in Biochemical Sciences·A H West, A M Stock
Nov 24, 1999·Molecular Microbiology·R DuttaM Inouye
Jun 26, 2001·European Journal of Biochemistry·T HübschmannN Tandeau de Marsac
May 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jun LiuWilliam Margolin
Feb 11, 2005·Journal of Biomolecular Structure & Dynamics·Debashis Mukhopadhyay, Kottayil I Varughese
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Sandra Da ReJeffry B Stock
Oct 20, 2006·Journal of Bacteriology·Collin M Dyer, Frederick W Dahlquist
Apr 28, 2001·Journal of Bacteriology·J S Wright, R J Kadner
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sang-Youn ParkBrian R Crane
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SchusterR B Bourret
Jan 1, 2009·Advances and Applications in Bioinformatics and Chemistry : AABC·Koki TsukamotoKazuhiko Fukui
Nov 4, 2000·Expert Opinion on Investigational Drugs·M J Macielag, R Goldschmidt
Jul 17, 2004·Molecular Microbiology·K MuchováI Barák
May 17, 2006·Biochemical and Biophysical Research Communications·Zhen-Hong LiXiao-Kui Guo
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Feb 16, 1999·Cell·A M BilwesM I Simon
Dec 15, 2012·International Journal of Biological Macromolecules·Dae-Ro AhnSangYoun Park
Mar 2, 1999·Journal of Structural Biology·S Djordjevic, A M Stock
Jan 8, 1999·Journal of Molecular Biology·M SoláM Coll
Dec 29, 1998·The Journal of Biological Chemistry·A RichardsonC Georgopoulos
Apr 19, 2003·The Journal of Biological Chemistry·María E CastelliEleonora García Vescovi
Mar 22, 2012·Chemistry : a European Journal·Mantosh K SinhaEhud Keinan

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