Two breakpoint clusters at fragile site FRA3B form phased nucleosomes

Genome Research
David J Mulvihill, Yuh-Hwa Wang

Abstract

Fragile sites are gaps and breaks in metaphase chromosomes generated by specific culture conditions. Fragile site FRA3B is the most unstable site and is directly involved in the breakpoints of deletion and translocation in a wide spectrum of cancers. To learn about the general characteristics of common fragile sites, we investigated the chromatin structure of the FRA3B site. Because FRA3B spans several hundred kilobases, we focused our study on two breakpoint clusters found in FRA3B. Using various nucleases, we demonstrated that these two regions contain phased nucleosomes, regardless of treatment with aphidicolin. Because these regions are located in intron 4 of the FHIT gene, it is very interesting to observe phased nucleosomes over these regions, which are several hundred kilobases downstream from the promoter. Further, by using nucleosome assembly assays, we demonstrate that these two regions do not contain strong nucleosome positioning elements. These results suggest that other factors appear to cooperate with the DNA sequence of these regions to impart nucleosome phasing. This study provides the first information on the chromatin structure of breakpoint regions in a common fragile site. The observation of phased nucleosom...Continue Reading

References

Sep 1, 1991·Genetic Analysis, Techniques and Applications·G R Sutherland
Sep 19, 1989·Biochemistry·A SaraiR L Jernigan
Feb 11, 1988·Nucleic Acids Research·S A MillerH F Polesky
Apr 28, 1995·The Journal of Biological Chemistry·E W Englander, B H Howard
Feb 1, 1997·Human Molecular Genetics·F BoldogH A Drabkin
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·R S HansenS M Gartler
Nov 1, 1996·Somatic Cell and Molecular Genetics·D E Eberhart, S T Warren
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·D MishmarB Kerem
Jan 1, 1997·Biopolymers·A Travers, H Drew
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MimoriC M Croce
May 30, 2001·Trends in Genetics : TIG·R I Richards
Mar 7, 2002·The Journal of Biological Chemistry·Ying Ying Hsu, Yuh-Hwa Wang
Mar 21, 2002·Nature Reviews. Cancer·K Huebner, C M Croce
Jun 28, 2002·Cancer Cell·Asaf HellmanBatsheva Kerem
Sep 17, 2002·American Journal of Human Genetics·Bradford CoffeeDaniel Reines
Jan 16, 2003·Cell·Anne M CasperThomas W Glover

❮ Previous
Next ❯

Citations

Sep 17, 2013·PloS One·Laura W DillonYuh-Hwa Wang
Sep 23, 2014·Cellular and Molecular Life Sciences : CMLS·Alexandros G GeorgakilasVassilis G Gorgoulis
Oct 10, 2014·Cellular and Molecular Life Sciences : CMLS·Efrat Ozeri-GalaiBatsheva Kerem
Jun 17, 2010·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Yan Fei DengYong De Lu
Dec 25, 2009·Journal of Cancer Research and Clinical Oncology·Md Imtaiyaz HassanFaizan Ahmad
Apr 3, 2012·Trends in Genetics : TIG·Efrat Ozeri-GalaiBatsheva Kerem
Nov 19, 2011·Trends in Genetics : TIG·Michelle DebatisseOlivier Brison

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved