Two C-P lyase operons in Pseudomonas stutzeri and their roles in the oxidation of phosphonates, phosphite, and hypophosphite

Journal of Bacteriology
Andrea K White, William W Metcalf

Abstract

DNA sequencing and analysis of two distinct C-P lyase operons in Pseudomonas stutzeri WM88 were completed. The htxABCDEFGHIJKLMN operon encodes a hypophosphite-2-oxoglutarate dioxygenase (HtxA), whereas the predicted amino acid sequences of HtxB to HtxN are each homologous to the components of the Escherichia coli phn operon, which encodes C-P lyase, although homologs of E. coli phnF and phnO are absent. The genes in the htx operon are cotranscribed based on gene organization, and the presence of the intergenic sequences is verified by reverse transcription-PCR with total RNA. Deletion of the htx locus does not affect the ability of P. stutzeri to grow on phosphonates, indicating the presence of an additional C-P lyase pathway in this organism. To identify the genes comprising this pathway, a Deltahtx strain was mutagenized and one mutant lacking the ability to grow on methylphosphonate as the sole P source was isolated. A ca.-10.6-kbp region surrounding the transposon insertion site of this mutant was sequenced, revealing 13 open reading frames, designated phnCDEFGHIJKLMNP, which were homologous to the E. coli phn genes. Deletion of both the htx and phn operons of P. stutzeri abolishes all growth on methylphosphonate and amino...Continue Reading

References

May 1, 1978·Applied and Environmental Microbiology·T L FosterS J Helms
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Sep 5, 1986·Journal of Molecular Biology·B L Wanner
Aug 7, 1965·Journal of Bacteriology·G Malacinski, W A Konetzka
Jul 3, 1998·Applied Microbiology and Biotechnology·G M YakovlevaB L Wanner
Dec 10, 1999·Natural Product Reports·H Seto, T Kuzuyama
Jul 14, 2000·Nature·B Schink, M Friedrich
Mar 30, 2001·The Journal of Biological Chemistry·A M CostasW W Metcalf
Aug 1, 1960·Journal of Bacteriology·L E CASIDA

❮ Previous
Next ❯

Citations

Nov 6, 2007·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Joao V CastroMarco A Z Ayub
Oct 26, 2005·Journal of Molecular Evolution·Jinling HuangYing Xu
Jul 27, 2011·Applied Microbiology and Biotechnology·Alexey V SviridovAlexey A Leontievsky
Mar 1, 2006·Biochemistry·James C Errey, John S Blanchard
Jan 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Matthew A Pasek
Apr 14, 2009·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Andrey Ilatovskiy, Michael Petukhov
Feb 22, 2011·Applied and Environmental Microbiology·Esther SingerKatrina J Edwards
Feb 8, 2006·Applied and Environmental Microbiology·Sonya T Dyhrman, Sheean T Haley
Mar 7, 2006·Applied and Environmental Microbiology·Russell D MondsGeorge A O'Toole
May 25, 2013·Genome Announcements·Anja PoehleinDiliana D Simeonova
Nov 13, 2007·Journal of Bacteriology·Melanie A AdamsZongchao Jia
Aug 20, 2004·Journal of Bacteriology·Andrea K White, William W Metcalf
Nov 24, 2007·Annual Review of Microbiology·Andrea K White, William W Metcalf
Sep 13, 2014·Genome Announcements·Anja PoehleinDiliana D Simeonova
Mar 18, 2016·Journal of Phycology·Senjie LinWilliam G Sunda
Oct 19, 2011·Environmental Microbiology·Asunción MartínezSallie W Chisholm
May 29, 2012·Chemosphere·Gwenaël ImfeldSylvain Payraudeau
Aug 5, 2017·Plant Biotechnology Journal·V Mohan M AcharyPawan K Agrawal
Nov 25, 2017·Nature Communications·Claudine BissonAndrew Hitchcock
May 31, 2015·Applied and Environmental Microbiology·Jonna TeikariKaarina Sivonen
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Bjarne Hove-JensenBjarne Jochimsen
Dec 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Israel A FigueroaJohn D Coates
Feb 12, 2019·The ISME Journal·Alejandra Guadalupe Acosta-CortésJuan Francisco Villarreal-Chiu
Dec 4, 2019·Applied Biochemistry and Biotechnology·Natalia StosiekMagdalena Klimek-Ochab
Dec 21, 2018·The Science of the Total Environment·Fiona H M TangFederico Maggi
Jan 28, 2021·International Journal of Molecular Sciences·Juan Francisco Martín, Paloma Liras
Dec 6, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xiuzhong WangJuewen Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.