Callose, a beta-1,3-glucan that is widespread in plants, is synthesized by callose synthase. Arabidopsis thaliana contains a family of 12 putative callose synthase genes (GSL1-12). The role of callose and of the individual genes in plant development is still largely uncertain. We have now used TILLING and T-DNA insertion mutants (gsl1-1, gsl5-2 and gsl5-3) to study the role of two closely related and linked genes, GSL1 and GSL5, in sporophytic development and in reproduction. Both genes are expressed in all parts of the plant. Sporophytic development was nearly normal in gsl1-1 homozygotes and only moderately defective in homozygotes for either of the two gsl5 alleles. On the other hand, plants that were gsl1-1/+ gsl5/gsl5 were severely defective, with smaller leaves, shorter roots and bolts and smaller flowers. Plants were fertile when the sporophytes had either two wild-type GSL1 alleles, or one GSL5 allele in a gsl1-1 background, but gsl1-1/+ gsl5/gsl5 plants produced an extremely reduced number of viable seeds. A chromosome with mutations in both GSL1 and GSL5 rendered pollen infertile, although such a chromosome could be transmitted via the egg. As a result, it was not possible to obtain plants that were homozygous for mut...Continue Reading
Premature dissolution of the microsporocyte callose wall causes male sterility in transgenic tobacco
The Saccharomyces cerevisiae FKS1 (ETG1) gene encodes an integral membrane protein which is a subunit of 1,3-beta-D-glucan synthase
The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats
The STUD gene is required for male-specific cytokinesis after telophase II of meiosis in Arabidopsis thaliana
Abnormal callose response phenotype and hypersusceptibility to Peronospoara parasitica in defence-compromised arabidopsis nim1-1 and salicylate hydroxylase-expressing plants
The shoot apical meristem restores its symplasmic organization during chilling-induced release from dormancy
A putative plant homolog of the yeast beta-1,3-glucan synthase subunit FKS1 from cotton (Gossypium hirsutum L.) fibers
Uridine Diphosphate Glucose Metabolism and Callose Synthesis in Cultured Pollen Tubes of Nicotiana alata Link et Otto
Transcriptional profiling of Arabidopsis tissues reveals the unique characteristics of the pollen transcriptome
Electron tomographic analysis of post-meiotic cytokinesis during pollen development in Arabidopsis thaliana
Biochemical evidence linking a putative callose synthase gene with (1 --> 3)-beta-D-glucan biosynthesis in barley
The AtNACK1/HINKEL and STUD/TETRASPORE/AtNACK2 genes, which encode functionally redundant kinesins, are essential for cytokinesis in Arabidopsis
The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotiana alata using a (1→3)-β-glucan-specific monoclonal antibody
Precocious pollen germination in Arabidopsis plants with altered callose deposition during microsporogenesis
Root hair-specific disruption of cellulose and xyloglucan in AtCSLD3 mutants, and factors affecting the post-rupture resumption of mutant root hair growth
Characterization of callase (β-1,3-D-glucanase) activity during microsporogenesis in the sterile anthers of Allium sativum L. and the fertile anthers of A. atropurpureum
Cytological mechanism of pollen abortion resulting from allelic interaction of F1 pollen sterility locus in rice (Oryza sativa L.)
HrpN Ea-induced deterrent effect on phloem feeding of the green peach aphid Myzus persicae requires AtGSL5 and AtMYB44 genes in Arabidopsis thaliana
Preferential protection of protein interaction network hubs in yeast: evolved functionality of genetic redundancy
Mapping pachytene chromosomes of coffee using a modified protocol for fluorescence in situ hybridization
Synergistic activation of defense responses in Arabidopsis by simultaneous loss of the GSL5 callose synthase and the EDR1 protein kinase
Arabidopsis transcriptome changes in response to phloem-feeding silverleaf whitefly nymphs. Similarities and distinctions in responses to aphids
Callose synthase GSL7 is necessary for normal phloem transport and inflorescence growth in Arabidopsis
Tie-dyed2 encodes a callose synthase that functions in vein development and affects symplastic trafficking within the phloem of maize leaves
Oxylipins produced by the 9-lipoxygenase pathway in Arabidopsis regulate lateral root development and defense responses through a specific signaling cascade
GLUCAN SYNTHASE-LIKE8 and STEROL METHYLTRANSFERASE2 are required for ploidy consistency of the sexual reproduction system in Arabidopsis
Pollen development in Annona cherimola Mill. (Annonaceae). Implications for the evolution of aggregated pollen
Developmental evolution of flowering plant pollen tube cell walls: callose synthase (CalS) gene expression patterns
Expression analysis of genes for callose synthases and Rho-type small GTP-binding proteins that are related to callose synthesis in rice anther
BcMF8, a putative arabinogalactan protein-encoding gene, contributes to pollen wall development, aperture formation and pollen tube growth in Brassica campestris
A novel lily anther-specific gene encodes adhesin-like proteins associated with exine formation during anther development
Genes Encoding Callose Synthase and Phytochrome A Are Adjacent to a MAP3Kα-Like Gene in Beta vulgaris US H20
Callose biosynthesis in Arabidopsis with a focus on pathogen response: what we have learned within the last decade
Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings
Expression of Arabidopsis callose synthase 5 results in callose accumulation and cell wall permeability alteration
The Arabidopsis glutathione transferase gene family displays complex stress regulation and co-silencing multiple genes results in altered metabolic sensitivity to oxidative stress
Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in male gametophyte development and plant growth
The GhACS1 gene encodes an acyl-CoA synthetase which is essential for normal microsporogenesis in early anther development of cotton
Novel demonstration of RNAi in citrus reveals importance of citrus callose synthase in defence against Xanthomonas citri subsp. citri
The microtubule-associated kinase-like protein RUNKEL functions in somatic and syncytial cytokinesis
Proteomic Analysis of a Poplar Cell Suspension Culture Suggests a Major Role of Protein S-Acylation in Diverse Cellular Processes
Traffic monitors at the cell periphery: the role of cell walls during early female reproductive cell differentiation in plants
GLUCAN SYNTHASE-LIKE 5 (GSL5) plays an essential role in male fertility by regulating callose metabolism during microsporogenesis in rice
The serine/threonine kinase 33 is present and expressed in palaeognath birds but has become a unitary pseudogene in neognaths about 100 million years ago
Combined microscopy and molecular analyses show phloem occlusions and cell wall modifications in tomato leaves in response to 'Candidatus Phytoplasma solani'
Down-regulation of the glucan synthase-like 6 gene (HvGsl6) in barley leads to decreased callose accumulation and increased cell wall penetration by Blumeria graminis f. sp. hordei
Cytological and biophysical comparative analysis of cell structures at the microsporogenesis stage in sterile and fertile Allium species
Increased callose deposition in plants lacking DYNAMIN-RELATED PROTEIN 2B is dependent upon POWDERY MILDEW RESISTANT 4
Novel localization of callose in the spores of Physcomitrella patens and phylogenomics of the callose synthase gene family
The conserved cysteine-rich domain of a tesmin/TSO1-like protein binds zinc in vitro and TSO1 is required for both male and female fertility in Arabidopsis thaliana
Quantitative proteomics reveals that plasma membrane microdomains from poplar cell suspension cultures are enriched in markers of signal transduction, molecular transport, and callose biosynthesis
Evolutionary history of callose synthases in terrestrial plants with emphasis on proteins involved in male gametophyte development
Exposure to heavy metal stress triggers changes in plasmodesmatal permeability via deposition and breakdown of callose
Correlation between pollen aperture pattern and callose deposition in late tetrad stage in three species producing atypical pollen grains
Relative importance of density-dependent regulation and environmental stochasticity for butterfly population dynamics
Genome-wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)
3-Aminobenzamide Blocks MAMP-Induced Callose Deposition Independently of Its Poly(ADPribosyl)ation Inhibiting Activity
Characterization of Callose Deposition and Analysis of the Callose Synthase Gene Family of Brassica napus in Response to Leptosphaeria maculans
Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis
Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer
Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.)
Expression Quantitative Trait Loci (eQTL) mapping for callose synthases in intergeneric hybrids of Citrus challenged with the bacteria Candidatus Liberibacter asiaticus
CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew
Transcriptome profiling of differentially expressed genes in floral buds and flowers of male sterile and fertile lines in watermelon
Characterization of black spot resistance in diploid roses with QTL detection, meta-analysis and candidate-gene identification
Comparative transcriptome analysis and ChIP-sequencing reveals stage-specific gene expression and regulation profiles associated with pollen wall formation in Brassica rapa
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