Two cases of maternal alloimmunization against human neutrophil alloantigen-4b, one causing severe alloimmune neonatal neutropenia

Transfusion
B R CurtisLee Ann Weitekamp

Abstract

Human neutrophil antigen (HNA)-4a/4bw is encoded by 230G>A in ITGAM, which results in an Arg61His substitution of the αM chain (CD11b) of complement receptor 3 (CR3; CD11b/18 or Mac-1). HNA-4a antibodies have been detected in the sera of female blood donors and in maternal sera that caused alloimmune neonatal neutropenia (ANN), in which maternal immunoglobulin (Ig)G antibodies against a paternally inherited HNA cross the placenta and destroy fetal and neonatal neutrophils. However, to date, antibodies specific for HNA-4b have not been reported. Here, we report the first two examples of HNA-4b antibodies. The two sera studied were both from previously pregnant females, one a multiparous female blood donor implicated in two separate transfusion reactions and the second a mother whose first pregnancy resulted in the birth of a severely neutropenic (0 × 10(6) neutrophils/L) infant affected with ANN. Serum neutrophil antibody testing was by flow cytometry and CD11b/18 monoclonal antibody immobilization of granulocyte antigens assay, and HNA genotyping was performed by polymerase chain reaction with sequence-specific priming and allele-specific 5' exonuclease assays. Sera from both women contained IgG antibodies reactive only with HN...Continue Reading

References

Feb 18, 1989·Lancet·C Mueller-EckhardtS Santoso
Jan 1, 1986·Vox Sanguinis·W E KlineJ McCullough
Sep 15, 2000·Clinics in Perinatology·R D ChristensenL M Rimsza
Feb 13, 2003·Transfusion Medicine·Y L FungR M Minchinton
Feb 21, 2006·Journal of Paediatrics and Child Health·Bronwyn A Williams, Y L Fung
Oct 28, 2010·Transfusion·Jerome L GottschallUNKNOWN NHLBI Retrovirus Epidemiology Donor Study II
Sep 14, 2011·Current Opinion in Hematology·Stefan MuschterAndreas Greinacher
May 29, 2012·Early Human Development·A Del Vecchio, R D Christensen
Sep 22, 2012·Journal of Pediatric Hematology/oncology·Sofia ÁguedaHercília Guimarães
Feb 20, 2014·American Journal of Reproductive Immunology : AJRI·Maja TomicicIrena Jukic
Apr 30, 2014·Clinical Chemistry·Hervé DelacourPatrick Imbert
Jul 22, 2014·Acta Paediatrica·Rita van den Tooren-de GrootLeendert Porcelijn

❮ Previous
Next ❯

Citations

Apr 5, 2016·Transfusion·Brigitte K FleschUlrich J Sachs
Dec 27, 2016·Transfusion·Pearl ToyMark R Looney
Jun 8, 2017·Transfusion Medicine·P SimtongA V Romphruk
Dec 28, 2017·Transfusion Medicine·K TamJ Kwok
May 20, 2020·Journal of Clinical Medicine·Borros Arneth
Dec 2, 2018·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Brigitte Katharina Flesch, Angelika Reil
May 3, 2019·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Leendert Porcelijn, Masja de Haas
Feb 3, 2021·British Journal of Haematology·Samira A AbbasJosé O Bordin

❮ Previous
Next ❯

Related Concepts

Related Feeds