Two Chitotriose-Specific Lectins Show Anti-Angiogenesis, Induces Caspase-9-Mediated Apoptosis and Early Arrest of Pancreatic Tumor Cell Cycle

PloS One
Ruby SinghC G Suresh

Abstract

The antiproliferative activity of two chito-specific agglutinins purified from Benincasa hispida (BhL) and Datura innoxia (DiL9) of different plant family origin was investigated on various cancer cell lines. Both lectins showed chitotriose specificity, by inhibiting lectin hemagglutinating activity. On further studies, it was revealed that these agglutinins caused remarkable concentration-dependent antiproliferative effect on human pancreatic cancerous cells but not on the normal human umbilical vein endothelial cells even at higher doses determined using MTT assay. The GI50 values were approximately 8.4 μg ml(-1) (0.247 μM) and 142 μg ml(-1) (14.8 μM) for BhL and DiL9, respectively, against PANC-1 cells. The growth inhibitory effect of these lectins on pancreatic cancer cells were shown to be a consequence of lectin cell surface binding and triggering G0/G1 arrest, mitochondrial membrane depolarization, sustained increase of the intracellular calcium release and the apoptotic signal is amplified by activation of caspases executing cell death. Interestingly, these lectins also showed anti-angiogenic activity by disrupting the endothelial tubulogenesis. Therefore, we report for the first time two chito-specific lectins specific...Continue Reading

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Citations

Oct 30, 2016·International Journal of Biological Macromolecules·Vinicius Jose Silva OsterneBenildo Sousa Cavada
Jul 4, 2017·International Journal of Molecular Sciences·Laura Elena Estrada-MartínezTeresa García-Gasca
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Aug 27, 2018·International Journal of Biological Macromolecules·Ana Paula M NascimentoRodrigo B Leal

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Methods Mentioned

BETA
glycosylation
gel filtration
Assay
PMA
confocal microscopy

Software Mentioned

Scientific High Content Screening ( HCS ) Studio
SkanIt
Thermo
HCS

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