Oct 31, 2018

Two Chloroflexi classes independently evolved the ability to persist on atmospheric hydrogen and carbon monoxide

BioRxiv : the Preprint Server for Biology
Zahra F IslamChris Greening

Abstract

Bacteria within aerated environments often exist within a variety of dormant forms. In these states, bacteria endure adverse environmental conditions such as organic carbon starvation by decreasing metabolic expenditure and using alternative energy sources. In this study, we investigated the energy sources that facilitate the persistence of the environmentally widespread but understudied bacterial phylum Chloroflexi. A transcriptome study revealed that Thermomicrobium roseum (class Chloroflexia) extensively remodels its respiratory chain upon entry into stationary phase due to organic carbon limitation. Whereas primary dehydrogenases associated with heterotrophic respiration were downregulated, putative operons encoding enzymes involved in molecular hydrogen (H2), carbon monoxide (CO), and sulfur compound oxidation were significantly upregulated. Gas chromatography and microsensor experiments were used to show that T. roseum aerobically respires H2 and CO at a range of environmentally relevant concentrations to sub-atmospheric levels. Phylogenetic analysis suggests that the enzymes mediating atmospheric H2 and CO oxidation, namely group 1h [NiFe]-hydrogenases and type I carbon monoxide dehydrogenases, are widely distributed in ...Continue Reading

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Mentioned in this Paper

Microorganism
Metabolic Process, Cellular
Study
Trichothecium roseum (fungus)
Genome
Enzymes, antithrombotic
Energy Metabolism
Cell Respiration
Sulfur Compounds
Phylogenetic Analysis

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