Two chlorophyll-binding subunits of the photosystem 2 reaction center complex isolated from the thermophilic cyanobacterium Synechococcus sp

Archives of Biochemistry and Biophysics
A Yamagishi, S Katoh

Abstract

The reaction center of photosystem 2 has been highly purified from digitonin-solubilized thylakoid membranes of the thermophilic cyanobacterium Synechococcus sp. by means of sucrose density gradient centrifugation and electrophoresis on polyacrylamide gels containing digitonin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of isolated reaction center complex yielded four chlorophyll a proteins named CP2-a, CP2-b, CP2-c, and CP2-d. When reelectrophoresed, CP2-a was transformed to CP2-d, and CP2-b was converted to CP2-a and CP2-d. The reaction center complex consisted of two major polypeptides of 47,000 and 40,000 Da and several minor polypeptides. CP2-b contained a 47,000-Da polypeptide together with 66,000- and 31,000-Da polypeptides, while CP2-a and CP2-d had only a 47,000-Da polypeptide. The apoprotein of CP2-c was a 40,000-Da polypeptide. Absorption spectra of CP2-a, -b, and -d were similar to each other but distinctly different from those of CP2-c at liquid nitrogen temperature. The reaction center complex showed two fluorescence emission bands at 686 and 694 nm at 77 degrees K. CP2-a, -b, and -d emitted the band at 694 nm, whereas the fluorescence peak at 686 nm was associated with CP2-c. It is concluded that t...Continue Reading

References

Jun 5, 1979·Biochimica Et Biophysica Acta·T Katoh, E Gantt
Mar 15, 1979·Biochimica Et Biophysica Acta·C P RijgersbergJ A Swager
Dec 17, 1979·European Journal of Biochemistry·J C Waldron, J M Anderson
Apr 28, 1978·Biochemical and Biophysical Research Communications·F Henriques, R B Park
Jul 6, 1978·Biochimica Et Biophysica Acta·J S Wessels, M T Borchert
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N H Chua, P Bennoun
Jun 30, 1970·Biochimica Et Biophysica Acta·F Cho, Govindjee
Feb 7, 1966·Biochimica Et Biophysica Acta·T OgawaK Shibata
Nov 1, 1982·Archives of Biochemistry and Biophysics·Y Takahashi, S Katoh
Nov 1, 1982·Archives of Biochemistry and Biophysics·Y TakahashiS Katoh
Apr 13, 1981·Biochimica Et Biophysica Acta·J E Mullet, C J Arntzen
Sep 1, 1980·European Journal of Biochemistry·B A Diner, F A Wollman
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Delepelaire, N H Chua
Feb 1, 1980·Plant Physiology·M Rusckowski, B A Zilinskas

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Citations

Jan 1, 1985·Photosynthesis Research·D F Ghanotakis, C F Yocum
Jan 1, 1986·Photosynthesis Research·K Satoh
Aug 1, 1986·Journal of Bioenergetics and Biomembranes·G S Bullerjahn, L A Sherman
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H B PakrasiL A Sherman
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S T WorlandJ E Hearst
Aug 1, 1985·Photochemistry and Photobiology· GovindjeeW Coleman

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