Two classes of proteins and mRNAs in Lilium longiflorum L. indentified by human vitronectin probes

Plant Physiology
C S WangE M Lord

Abstract

Vitronectin (VN) is a substrate adhesion molecule, an extracellular matrix glycoprotein that facilitates cell adhesion and cell movement in animals. We have reported the cross-reactivity of a 55-kD protein in plants with rabbit anti-human VN antibodies and the presence of VN-like sequences in plant genomes using a human VN cDNA probe. We have extended these studies by using human VN riboprobes to detect VN-like mRNAs in lily (Lilium longiflorum L.) and soybean. In both species, two mRNAs were detected. We have also identified a new cross-reactive protein (41 kD) using a different preparation of human VN antiserum. In lily roots five 41-kD isoforms were observed, whereas only three of these isoforms accumulated in leaves. Monospecific antibodies prepared against the plant proteins cross-reacted with the human VN protein and vice versa. We have purified the 41-kD protein using two-dimensional gel electrophoresis, and amino acid composition analysis indicates that it is similar in composition to human VN.

References

Feb 21, 1978·Biochemistry·C E RozekW E Timberlake
Jan 1, 1991·Annual Review of Cell Biology·K T Preissner
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S MeinersM Schindler
Oct 1, 1990·Current Opinion in Cell Biology·K Roberts
Aug 1, 1988·Cell Structure and Function·T YatohgoM Hayashi
May 1, 1989·The Journal of Cell Biology·M SchindlerD A Cheresh
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
May 17, 1988·Biochemistry·G M Edelman
Jun 4, 1974·Biochemistry·V GlisinC Byus
Sep 1, 1993·The Plant Cell·A R Mushegian, E V Koonin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.