Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells

Nanotheranostics
Jing LiKemin Wang

Abstract

MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical diagnoses. Simultaneous detection of multiple biomarkers could effectively improve the accuracy of early cancer diagnosis. Here, we develop the two-color-based nanoflares for simultaneously detecting two distinct miRNA targets inside live cells. The nanoflares consist of gold nanoparticles (AuNPs) functionalized with a dense shell of recognition sequences hybridized to two short fluorophore-labeled DNA molecules, termed "flares". In this conformation, the close proximity of the fluorophore to the AuNPs surface leads to quenching of the fluorescence. However, when target miRNAs bind to the recognition sequence, the concomitant displacement of the flare can be detected as a corresponding increase in fluorescence. The results demonstrate that the two-color-based nanoflares can simultaneously detect miR-21 and miR-141 expression levels in various live cancer cells successfully....Continue Reading

Citations

Dec 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dinggeng HeHung-Wing Li
Feb 5, 2021·Chemical Society Reviews·Thomas JetValérie Taly
Dec 12, 2018·ACS Applied Materials & Interfaces·Maria MorosAntonios G Kanaras
Aug 27, 2021·ACS Applied Materials & Interfaces·Peng GaoBo Tang
Sep 4, 2019·Bioconjugate Chemistry·Hao WangLaura Fabris

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Methods Mentioned

BETA
Flow cytometry
fluorescence imaging

Software Mentioned

image proplus
Image J
Light Cycler480

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