PMID: 3746249Aug 1, 1986Paper

Two components of voltage-dependent calcium influx in mouse neuroblastoma cells. Measurement with arsenazo III

The Journal of General Physiology
S R Bolsover

Abstract

N1E-115 mouse neuroblastoma cells were injected with the calcium indicator dye arsenazo III. Optical absorbance changes during voltage-clamp depolarization were used to examine the properties of the two calcium currents present in these cells. The rapidly inactivating calcium current (Moolenar and Spector, 1979b, Journal of Physiology, 292:307-323) inactivates by a voltage-dependent mechanism. The slowly inactivating calcium current is dominant in raising intracellular calcium during depolarizations to greater than -20 mV. Lowering the extracellular calcium concentration affects the two calcium currents unequally, with the slowly inactivating current being reduced more. Intracellular calcium falls very slowly (tau greater than 1 min) after a depolarization. The rapidly inactivating calcium current is responsible for a calcium action potential under physiological conditions. In contrast, it is unlikely that the slowly inactivating calcium current has an important electrical role. Rather, its function may be to add a further increment of calcium influx over and above the calcium influx through the rapidly inactivating calcium channels.

Citations

Mar 1, 1996·The European Journal of Neuroscience·F Zimprich, S R Bolsover
Dec 5, 2013·Physiological Reports·Stewart O SageAlan G S Harper
Jul 1, 1988·Pflügers Archiv : European journal of physiology·S A ThayerR J Miller

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