Abstract
We have recently discovered that the Masculinizer (Masc) gene encodes a CCCH tandem zinc finger protein, which controls both masculinization and dosage compensation in the silkworm Bombyx mori. In this study, we attempted to identify functional regions or residues that are required for the masculinizing activity of the Masc protein. We constructed a series of plasmids that expressed the Masc derivatives and transfected them into a B. mori ovary-derived cell line, BmN-4. To assess the masculinizing activity of the Masc derivatives, we investigated the splicing patterns of B. mori doublesex (Bmdsx) and the expression levels of B. mori IGF-II mRNA-binding protein, a splicing regulator of Bmdsx, in Masc cDNA-transfected BmN-4 cells. We found that two zinc finger domains are not required for the masculinizing activity. We also identified that the C-terminal 288 amino acid residues are sufficient for the masculinizing activity of the Masc protein. Further detailed analyses revealed that two cysteine residues, Cys-301 and Cys-304, in the highly conserved region among lepidopteran Masc proteins are essential for the masculinizing activity in BmN-4 cells. Finally, we showed that Masc is a nuclear protein, but its nuclear localization is...Continue Reading
References
Nov 11, 1994·Nucleic Acids Research·J D ThompsonT J Gibson
Oct 1, 1996·Nature Biotechnology·F YangG N Phillips
May 4, 2002·Science·David A ZachariasRoger Y Tsien
May 7, 2003·Development Genes and Evolution·Masataka G SuzukiToru Shimada
Jan 12, 2005·Evolution & Development·Masataka G SuzukiToru Shimada
Aug 12, 2005·Cytogenetic and Genome Research·H AbeT Shimada
Sep 29, 2007·Genetica·H AbeT Shimada
May 23, 2009·RNA·Shinpei KawaokaSusumu Katsuma
Oct 27, 2009·Molecular Biology and Evolution·Manolo GouyOlivier Gascuel
Nov 10, 2009·Plant Physiology·Marcelo C PomeranzJyan-Chyun Jang
Jun 18, 2010·The Journal of Biological Chemistry·Seth M KellyAnita H Corbett
Oct 20, 2010·Molecular and Cellular Biology·Masataka G SuzukiShogo Matsumoto
Jun 2, 2011·RNA·Shinpei KawaokaSusumu Katsuma
Oct 25, 2011·RNA·Shinpei KawaokaSusumu Katsuma
Feb 23, 2013·Biochimica Et Biophysica Acta·Seth A Brooks, Perry J Blackshear
Mar 20, 2013·Virus Research·Genki IshiharaSusumu Katsuma
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 19, 2014·The Plant Journal : for Cell and Molecular Biology·Jie QuJyan-Chyun Jang
Apr 5, 2014·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Perry J Blackshear, Lalith Perera
May 16, 2014·Nature·Takashi KiuchiSusumu Katsuma
Jan 24, 2015·Gene·Munetaka KawamotoSusumu Katsuma
Mar 10, 2015·Annual Review of Biochemistry·Yuka W IwasakiHaruhiko Siomi
Jul 15, 2015·Insect Molecular Biology·J LeeS Katsuma
Jul 15, 2015·PLoS Pathogens·Takahiro FukuiSusumu Katsuma
Citations
Jun 10, 2016·FEBS Letters·Yudai SuganoSusumu Katsuma
Sep 1, 2016·PLoS Genetics·Hiroki SakaiMasataka G Suzuki
Jan 30, 2018·ELife·Yu FuPhillip D Zamore
Aug 9, 2018·Insect Science·Yao-Hui WangShuai Zhan
Mar 7, 2019·RNA Biology·Zeng-Zhang ZhengYong-Zhen Xu
Dec 4, 2019·Insect Molecular Biology·T Harvey-SamuelL Alphey
Feb 24, 2018·PLoS Genetics·Zhiqian LiAnjiang Tan
Jul 12, 2019·FEBS Open Bio·Susumu KatsumaTakashi Kiuchi
Aug 2, 2019·Biomolecules·Qin ZhaoXing-Fu Zha
May 16, 2018·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Susumu KatsumaKen Sahara
Sep 23, 2020·BMC Developmental Biology·Tomohisa YuzawaMasataka G Suzuki
Apr 12, 2020·International Journal of Molecular Sciences·Yao WangXing-Fu Zha
Mar 7, 2021·Genes·Xu YangYongping Huang
Apr 13, 2021·Pest Management Science·Yaohui WangYongping Huang
Aug 3, 2021·PLoS Genetics·Sander VisserFrantišek Marec
Aug 28, 2021·International Journal of Molecular Sciences·Zhongyuan DengXianchun Li