Two Convenient Methods to Evaluate Soybean for Resistance to Sclerotinia sclerotiorum

Plant Disease
Y Chen, D Wang

Abstract

Several greenhouse inoculation methods are available to evaluate soybean (Glycine max (L.) Merr.) for resistance to Sclerotinia sclerotiorum (Lib.) de Bary. Most of these methods are labor intensive and often produce inconsistent results among the tests. The objective of this research was to develop a low-cost and high-efficiency greenhouse inoculation method that can generate a consistent result. We developed a spray-mycelium method in which mycelia were cultured in liquid potato dextrose broth and homogenized before spraying on the soybean leaves. We also developed an inoculation method (the "drop-mycelium" method) in which a drop of homogenized mycelium suspension was dropped on the tips of main stems. Inoculated plants were incubated in a greenhouse chamber with 60 to 80% relative humidity. Plant mortality and area under the wilt progress curve (AUWPC) were used to measure disease severity daily from 3 to 14 days after inoculation (DAI). Eighteen soybean genotypes, including partially resistant line NKS19-90 and susceptible line Resnik, were employed in this study. The spray-mycelium method and the drop-mycelium method were compared with the cut-petiole method in the greenhouse. The three experiments were a randomized compl...Continue Reading

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Nov 5, 2019·Frontiers in Plant Science·Zheng WangXiao-Li Tan
Feb 6, 2021·The Plant Journal : for Cell and Molecular Biology·Diego RebaqueHugo Mélida
Jun 3, 2021·Journal of Fungi·Alejandro Moreno-GavíraMila Santos

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