Two copies of blaNDM-1 gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia

Antonie van Leeuwenhoek
Branko JovcićMilan Kojić

Abstract

New Delhi metallo-β-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the blaNDM-1 gene. Restriction analysis and hybridization revealed that two copies of the blaNDM-1 gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the blaNDM-1 gene in the genome of P. aeruginosa MMA83.

References

Apr 10, 1995·Analytical Biochemistry·B M BartonA J Zuccarelli
Sep 21, 2007·Journal of Clinical Microbiology·Jennifer K JohnsonAnthony D Harris
Dec 15, 2010·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·M J StruelensUNKNOWN European NDM-1 Survey Participants
Jun 8, 2011·Antimicrobial Agents and Chemotherapy·Branko JovcicMilan Kojic
Nov 15, 2011·Trends in Microbiology·Patrice NordmannDavid M Livermore
Apr 25, 2013·Antimicrobial Agents and Chemotherapy·Branko JovcićMilan Kojić
Apr 25, 2013·Antimicrobial Agents and Chemotherapy·Frédéric JanvierAudrey Mérens

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