Two delta-crystallin polypeptides are derived from a cloned delta 1-crystallin cDNA

FEBS Letters
E F WawrousekJ Piatigorsky

Abstract

Previous studies have shown that there are 2 similar delta-crystallin genes (delta 1 and delta 2) and at least 2 delta-crystallin polypeptides in the chicken eye lens. We show here that both delta-crystallin polypeptides can be synthesized from mRNA transcribed in vitro from a cloned delta 1-crystallin cDNA. Both polypeptides co-migrate in SDS-urea-polyacrylamide electrophoresis with their authentic counterparts isolated from 15-day-old embryonic chicken lenses, and both react with sheep anti-chicken delta-crystallin serum. Screening nearly 900 delta-crystallin cDNA clones from a 15-day-old embryonic lens library with an oligonucleotide probe specific for exon 2 of the delta 2-crystallin gene failed to detect any delta 2 cDNA clones, indicating that the delta 2 gene produces little or no mRNA in the lens at this stage of development. Our results suggest that both of the observed delta-crystallin polypeptides are derived from mRNA transcribed from the delta 1 gene, with heterogeneity arising at the translational or co-translational level.

References

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Citations

May 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R J BuonoJ Piatigorsky
Jan 1, 1987·Journal of Molecular Evolution·J PiatigorskyR E Jones
Apr 1, 1988·Developmental Biology·D S ParkerJ Piatigorsky
Mar 1, 1989·Developmental Biology·P S ZelenkaM Vatal
May 1, 1990·Developmental Biology·C M SaxJ Piatigorsky
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L S YehW C Barker
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PiatigorskyE F Wawrousek
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G WistowJ Piatigorsky
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Apr 1, 1988·Current Eye Research·G C Das, J Piatigorsky
Jul 1, 1989·Molecular and Cellular Biology·C H SullivanR M Grainger

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