Two different genogroups of Ehrlichia canis from dogs in Thailand using immunodominant protein genes

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Boondarika NamboopphaNattawooti Sthitmatee

Abstract

Ehrlichia canis is the causative agent of canine monocytic ehrlichiosis (CME). While there is a high prevalence of CME in Thailand, genetic diversity of E. canis is still poorly defined. This study examined the molecular characteristics of E. canis using PCR and phylogenetic analysis of the dsb, gp19 and gp36 genes. DNA was extracted from 220 whole blood samples of naturally infected dogs, and all had clinical signs compatible with tick-borne diseases. Of these, 16.4% (36/220) provided positive E. canis DNA via the dsb and gp19 genes. However, only 13 out of the 36 samples (36.1%) were positive for the gp36 gene. Sequences of the dsb gene had very high identity (99-100%) with previously deposited E. canis sequences. Sequences of the gp19 gene were similar to those from US and Taiwanese genogroups (98.8-99.5% identity). Elucidation of genetic characteristics of E. canis based on the gp36 gene displayed 91.4-99.1% shared identity. There were 426-429 bp of a 5' end pre-repeat tandem region, a 27 bp repetition with variable numbers of a tandem repeat (TR) region of 9 amino acid sequences (TEDSVSAPA), and a variable 3' end region with sequence length depending on the isolate (72-93 bp). Phylogenetic trees of E. canis, particularly u...Continue Reading

Citations

Aug 28, 2020·Veterinary World·Thanakorn Rawangchue, Sivapong Sungpradit
Mar 20, 2021·Veterinary Microbiology·Marcos Antônio Bezerra-SantosDomenico Otranto
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Boondarika NamboopphaNattawooti Sthitmatee

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