Two different mechanisms underlie reversible, intrinsic optical signals in rat hippocampal slices

Journal of Neurophysiology
Dmitriy FayukDennis A Turner

Abstract

Intrinsic optical signals (IOSs) induced by synaptic stimulation and moderate hypotonic swelling in brain tissue slices consist of reduced light scattering and are usually attributed to cell swelling. During spreading depression (SD), however, light-scattering increases even though SD has been shown to cause strong cell swelling. To understand this phenomenon, we recorded extracellular voltage, light transmission (LT), which is inversely related to light scattering, and interstitial volume (ISV) simultaneously from the same site (stratum radiatum of CA1) in both interface and submerged hippocampal slices. As expected, moderate lowering of bath osmolarity caused concentration-dependent shrinkage of ISV and increase in LT, while increased osmolarity induced opposite changes in both variables. During severe hypotonia, however, after an initial increase of LT, the direction of the IOS reversed to a progressive decrease in spite of continuing ISV shrinkage. SD caused by hypotonia, by microinjection of high-K(+) solution, or by hypoxia, was associated with a pronounced LT decrease, during which ISV shrinkage indicated maximal cell swelling. If most of the extracellular Cl(-) was substituted by the impermeant anion methylsulfate and a...Continue Reading

References

Dec 12, 1991·Biochimica Et Biophysica Acta·B Sarkadi, J C Parker
Jul 1, 1966·Journal of Neurophysiology·H Martins-Ferreira, G O de Castro
Apr 1, 1980·Acta Physiologica Scandinavica·A J Hansen, C E Olsen
Sep 1, 1994·Neuroscience·R D Andrew, B A MacVicar
Oct 1, 1994·Experimental Neurology·N Hori, D O Carpenter
Apr 1, 1996·Journal of Neuroscience Methods·D MelzianF Tegtmeier
Feb 1, 1997·Experimental Neurology·R D AndrewE P Osehobo
Nov 5, 1997·Trends in Neurosciences·A Villringer, B Chance
Oct 7, 1998·Epilepsy Research·P A SchwartzkroinD W Hochman
Jan 23, 1999·Journal of Neurophysiology·D W HochmanP A Schwartzkroin
Jun 5, 1999·Methods : a Companion to Methods in Enzymology·P G AitkenD A Turner
Jun 5, 1999·Methods : a Companion to Methods in Enzymology·T J Sick, M A Perez-Pinzon
Jun 5, 1999·Methods : a Companion to Methods in Enzymology·R D AndrewA S Obeidat
Jul 13, 1999·Journal of Neurophysiology·N R Kreisman, J C LaManna
Jan 15, 2000·Journal of Neurophysiology·D W Hochman, P A Schwartzkroin
Jan 12, 2001·Journal of Neurophysiology·I Joshi, R D Andrew
Apr 18, 2001·Brain Research·I VilágiH J Luhmann

❮ Previous
Next ❯

Citations

Oct 15, 2013·The European Journal of Neuroscience·Sándor BorbélyIldikó Világi
Jun 26, 2007·NeuroImage·K HolthoffO W Witte
Apr 6, 2007·Journal of Magnetic Resonance Imaging : JMRI·Ute Goerke, Harald E Möller
Aug 26, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Francesca GaleffiDennis A Turner
Dec 25, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hellmuth Obrig, Arno Villringer
May 13, 2004·Journal of Neuroscience Research·Ryuichi NakajimaYoshihisa Kudo
Apr 22, 2003·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Timothy M ShepherdStephen J Blackband
Jan 29, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·C van PulP F F Wijn
May 3, 2005·The Journal of Biological Chemistry·Harshad D VishwasraoWatt W Webb
Jan 11, 2003·Journal of Neurophysiology·Michael Müller, Klaus Ballanyi
Mar 6, 2009·Journal of Neurophysiology·Frank FunkeMichael Müller
Mar 4, 2021·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Dennis A TurnerCarol A Colton
Aug 28, 2019·Biosensors & Bioelectronics·Shuangjie LiuDong Ming
Apr 14, 2018·Experimental Neurology·Katelyn M Reinhart, C William Shuttleworth
Jul 6, 2021·Aging and Disease·Dennis A Turner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.