PMID: 8979045Nov 1, 1996Paper

Two different patterns of airway branching regulated by different components of the extracellular matrix in vitro

Experimental Lung Research
R MatsuiH S Sekhon

Abstract

This study examined the effects of beta-D-xyloside (an inhibitor of proteoglycan synthesis) and cis-4-hydroxyl-L-proline (an inhibitor of collagen synthesis) on branching morphogenesis in cultures of fetal rat lung. Lungs from day 15 gestation were incubated for 4 days in (1) the control medium (Dulbecco's Modified Eagle Medium + 10% Fetal Bovine Serum) alone (control), (2) control medium plus 2 mM beta-D-xyloside (beta-XYL), (3) control medium plus 2 mM alpha-D-xyloside (alpha-XYL), (4) control medium plus 50 micrograms/mL cis-4-hydroxy-L-proline (cis-HYP). The number of peripheral buds of left lungs was counted daily. Histological examination was performed on lungs on days 2 and 4. beta-XYL inhibited proximal monopodial branching on day 2 without affecting lung size, but produced numerous peripheral buds on day 4 which were of abnormal appearance, suggesting that lung airway branching and growth may be regulated by different mechanisms. Histology and morphometry showed significantly enlarged airspaces and diminished mesenchyme. cis-HYP did not affect proximal branching on 2 days in culture, but inhibited further dichotomous branching and lung growth after 2 days. On day 4, diminished branching and lung growth was accompanied ...Continue Reading

References

Sep 1, 1978·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·I GrossS A Rooney
Mar 8, 1991·Cell·E Ruoslahti, Y Yamaguchi
Aug 1, 1988·Developmental Biology·B LelongtY S Kanwar
Jan 1, 1987·Experimental Lung Research·G M King, I Y Adamson
Jan 1, 1985·Tissue & Cell·S R HilferJ W Brown
Dec 1, 1970·The Journal of Experimental Zoology·B S Spooner, N K Wessells
Dec 1, 1970·The Journal of Experimental Zoology·N K Wessells
Nov 1, 1967·Developmental Biology·J V Taderera
Apr 1, 1982·Developmental Biology·M Bernfield, S D Banerjee
Jun 1, 1980·Developmental Biology·B S Spooner, J M Faubion
Feb 1, 1993·Pediatric Pulmonology·E A MassoudW M Thurlbeck

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Citations

Sep 27, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jamie A Davies
May 1, 2006·Environmental Toxicology and Pharmacology·Sohail Ejaz, Chae Woong Lim
Mar 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B YuanP Minoo
Mar 5, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jun LiA Keith Tanswell

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